Genetically Engineering Bacillus subtilis with a Heat-Resistant Arsenite Methyltransferase for Bioremediation of Arsenic-Contaminated Organic Waste
Genetically Engineering Bacillus subtilis with a Heat-Resistant Arsenite Methyltransferase for Bioremediation of Arsenic-Contaminated Organic Waste
复制标题
具有耐热亚砷酸甲基转移酶的基因工程枯草芽孢杆菌用于砷污染的有机废物的生物修复
DOI：
10.1128/aem.01535-15
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发表时间：
2015-10-01
影响因子：
4.4
通讯作者：
Zhao, Fang-Jie
中科院分区：
文献类型：
--
作者：
Huang, Ke;Chen, Chuan;Zhao, Fang-Jie
摘要由于在动物饲料中使用含砷促生长物质，有机肥可能含有高水平的砷。为了探索一种去除有机废物中砷的生物修复策略，利用基因工程技术从嗜热藻类蓝藻Merolae中表达了一株能够在高温下生长但不能甲基化和挥发砷的细菌枯草芽孢杆菌168。基因工程菌B.subtilis 168在48h内将培养基中的大部分无机砷转化为二甲基砷酸盐和三甲基砷氧化物，并挥发出大量的二甲基砷和三甲基砷。在37~50℃温度范围内，As甲基化和挥发速率随着温度的升高而增加，接种于50℃堆肥的As污染有机肥后，菌体的挥发能力显著增强。这项研究为利用基因工程微生物在堆肥过程中对受As污染的有机废物进行生物修复的概念提供了证据。
ABSTRACT Organic manures may contain high levels of arsenic (As) due to the use of As-containing growth-promoting substances in animal feed. To develop a bioremediation strategy to remove As from organic waste, Bacillus subtilis 168, a bacterial strain which can grow at high temperature but is unable to methylate and volatilize As, was genetically engineered to express the arsenite S-adenosylmethionine methyltransferase gene (CmarsM) from the thermophilic alga Cyanidioschyzon merolae. The genetically engineered B. subtilis 168 converted most of the inorganic As in the medium into dimethylarsenate and trimethylarsine oxide within 48 h and volatized substantial amounts of dimethylarsine and trimethylarsine. The rate of As methylation and volatilization increased with temperature from 37 to 50°C. When inoculated into an As-contaminated organic manure composted at 50°C, the modified strain significantly enhanced As volatilization. This study provides a proof of concept of using genetically engineered microorganisms for bioremediation of As-contaminated organic waste during composting.