The regulation of pinocytosis in mouse macrophages. 3. The induction of vesicle formation by nucleosides and nucleotides.
The regulation of pinocytosis in mouse macrophages. 3. The induction of vesicle formation by nucleosides and nucleotides.
复制标题
小鼠巨噬细胞中皮细胞增多症的调节。 3。核苷和核苷酸诱导囊泡形成。
DOI：
10.1084/jem.125.3.457
复制
发表时间：
1967-03-01
影响因子：
15.3
通讯作者：
Parks, E
中科院分区：
文献类型：
--
作者：
Cohn, Z A;Parks, E
研究了核苷和核苷酸对小鼠巨噬细胞胞饮小泡形成的影响。巨噬细胞暴露于腺苷5 '-磷酸、ADP和ATP后，发现极高水平的胞质囊泡。用腺苷2 '-获得有限的囊泡反应。和3 ′-磷酸、3 ′，5 ′-腺苷-磷酸和脱氧腺苷5 ′-磷酸。鸟苷、肌苷、胞苷和尿苷的二磷酸和三磷酸具有刺激性，但活性远低于腺苷衍生物。腺苷给药导致高水平的胞饮活性，而其他核苷，包括肌苷，鸟苷，胞苷和尿苷，产生很少或没有刺激作用。腺苷及其5 '-磷酸盐对巨噬细胞产生形态学影响，其特征在于扩展增加、薄的外周细胞质面纱，具有定向线粒体的致密核心和中心球区域的收缩。大量的胞饮体与细胞质中含有胞饮体的部分相关联。腺苷和ATP的刺激作用是迅速的，涉及培养物中的大多数细胞。腺苷不能逆转由2，4-DNP产生的胞饮抑制，而ATP使囊泡计数升高到高水平。这些影响的可能机制进行了讨论。
A study has been conducted on the influence of nucleosides and nucleotides to induce the formation of pinocytic vesicles in cultured mouse macrophages. Extremely high levels of cytoplasmic vesicles were found after exposure of macrophages to adenosine 5'-phosphate, ADP, and ATP. A limited vesicle response was obtained with adenosine 2'-. and 3'-phosphate, 3',5'-adenosine-phosphate, and deoxyadenosine 5'-phosphate. The di- and triphosphates of guanosine, inosine, cytidine, and uridine were stimulatory but much less active than the adenosine derivatives. Adenosine administration resulted in high levels of pinocytic activity whereas other nucleosides, including inosine, guanosine, cytidine, and uridine, yielded little or no stimulatory effect. Adenosine and its 5'-phosphates produced morphological effects on macrophages characterized by increased spreading, a thin, peripheral cytoplasmic veil with denser cores of oriented mitochondria and contraction of the centre-sphere region. Large numbers of pinosomes were seen in association with mitochondria-containing portions of the cytoplasm. The stimulatory effects of adenosine and ATP were rapid and involved the majority of cells in the culture. Adenosine was unable to reverse the pinocytosis inhibition produced by 2,4-DNP, whereas ATP raised vesicle counts to high levels. Possible mechanisms for these effects are discussed.