In vivo endoscopic mass spectrometry using a moving string sampling probe
In vivo endoscopic mass spectrometry using a moving string sampling probe
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使用移动弦采样探针进行体内内窥镜质谱分析
DOI：
10.1039/c7an00650k
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hiraoka Kenzo
中科院分区：
文献类型：
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作者：
Chen Lee Chuin;Naito Tsubasa;Tsutsui Satoru;Yamada Yuki;Ninomiya Satoshi;Yoshimura Kentaro;Takeda Sen;Hiraoka Kenzo
目前，内窥镜检查几乎完全依赖于光学显微镜，而准确的分析，如质谱分析，是通过活检进行的。在这项工作中，开发了一种新的探测系统，用于使用移动弦进行材料的取样和运输，以执行原位和体内内窥镜质谱分析。利用工业内窥镜和商用质谱仪构建了质谱内窥镜的原型。采样系统由一个由电动滑轮驱动的移动棉线组成。当取样探针触及目标表面时，棉线“擦拭”并将粘附的样品输送到离子源。根据目标分析物的不同，解吸电喷雾和大气压化学电离源可互换用于解吸和电离。被分析的表面不受热、有机溶剂、高压或带电液滴的影响。活体小鼠的原位内窥镜质谱分析和志愿者口腔内的表面分析。
At present, endoscopy relies almost exclusively on optical microscopy and the accurate analysis such as MS interrogation is performed ex situ using biopsy. In this work, a novel probing system is developed to perform in situ and in vivo endoscopic mass spectrometry using a moving string for the sampling and transportation of material. A prototype of a mass spectrometric endoscope is constructed using an industrial endoscope and a commercial mass spectrometer. The sampling system consists of a moving cotton thread driven by motorized pulleys. When the target surface is touched by the sampling probe, the cotton thread “wipes” and transports the adhered sample to the ion source. Depending on the target analytes, desorption electrospray and atmospheric pressure chemical ionization sources are employed interchangeably for the desorption and ionization. The surface under analysis is not subjected to heat, organic solvents, high voltage or charged droplets. In situ endoscopic MS of a living mouse and surface analysis inside a volunteer subject's mouth are demonstrated.