Rickettsia hoogstraalii sp nov., isolated from hardand soft-bodied ticks
Rickettsia hoogstraalii sp nov., isolated from hardand soft-bodied ticks
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DOI：
10.1099/ijs.0.011049-0
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发表时间：
2010-04-01
影响因子：
2.8
通讯作者：
Strus, Jasna
中科院分区：
文献类型：
--
作者：
Duh, Darja;Punda-Polic, Volga;Strus, Jasna
2006年在克罗地亚的绵羊和山羊身上发现了一种新的斑点热群立克次体。与此同时，在美国格鲁吉亚从软蜱Carios capensis获得的胚胎细胞系CCE 3中共分离出遗传上相同的生物体。本研究进一步研究了H.研究了沟形蜱。基于蚊子和Vero细胞培养物中细菌的培养，蜱组织和细胞培养物中立克次体的存在[通过透射电子显微镜（TEM）证实]以及5个立克次体基因的扩增和测序，证明新型立克次体菌株符合归类为新物种的标准。建议将该新菌株命名为Rickettsia hoogstraalii sp. nov.。胡氏立克次体，一种专性胞内细菌在Vero细胞和节肢动物CCE 3、ISE 6和C6/36细胞系中生长。新种的细胞形态是典型的SFG立克次体。在光学显微镜下，细菌的小球杆菌外观明显。通过TEM可以看到革兰氏阴性细菌的细胞壁和由狭窄的周质间隙隔开的细胞质膜。迄今为止，已从两种蜱中分离到霍氏立克次体，即霍氏立克次体。sulcata和C. Capensis。新物种似乎在地理上分布广泛，在克罗地亚，西班牙和格鲁吉亚，美国被发现。虽然没有关于新种对脊椎动物宿主可能的致病性的信息，但R。Hoogstraalii sp. nov.在Vero、CCE 3和ISE 6细胞中具有细胞病变效应。对16 SrRNA、17 kDa、gitA、ompA和ompB基因的序列分析表明，尽管R. hoogstraalii sp. nov.与Rickettsia fells亲缘关系较近，是斑点热组中的一个独立种。R. hoogstraalii sp. nov.为Croatica（T）菌株（=DSM 22243（T）=UTMB 00003（T））。
A novel spotted fever group Rickettsia was found in Haemaphysalis sulcata ticks collected from sheep and goats in Croatia in 2006. At the same time, a genetically identical organism was co-isolated with the embryonic cell line CCE3 obtained from the soft tick Carios capensis in Georgia, USA. In this study, further phenotypic and genotypic characteristics of the novel rickettsial strain present in H. sulcata ticks were investigated. Based on the cultivation of bacteria in mosquito and Vero cell cultures, the presence of rickettsiae in tick tissues and cell cultures [confirmed by transmission electron microscopy (TEM)] and the amplification and sequencing of five rickettsial genes, it was demonstrated that the novel Rickettsia strain fulfils the criteria to be classified as a novel species. The name Rickettsia hoogstraalii sp. nov. is proposed for the new strain. Rickettsia hoogstraalii sp. nov., an obligately intracellular bacterium, was grown in Vero cells and arthropod CCE3, ISE6 and C6/36 cell lines. The morphology of the cells of the novel species was typical of SFG rickettsiae. The small coccobacillary appearance of the bacteria was apparent with light microscopy. A Gram-negative bacterial cell wall and a cytoplasmic membrane separated by a narrow periplasmic space were visible by TEM. To date, Rickettsia hoogstraalii sp. nov. has been isolated from two species of ticks, H. sulcata and C. capensis. The novel species appears to be geographically widely distributed, having been detected in Croatia, Spain and Georgia, USA. Although no information is available regarding the possible pathogenicity of the novel species for vertebrate hosts, R. hoogstraalii sp. nov. has a cytopathic effect in Vero, CCE3 and ISE6 cells. Sequence analyses of the 16S rRNA, 17 kDa, gitA, ompA and ompB genes indicated that even though R. hoogstraalii sp. nov. was closely related to Rickettsia fells, it represents a separate species within the spotted fever group. The type strain of R. hoogstraalii sp. nov. is strain Croatica(T) (=DSM 22243(T)=UTMB 00003(T)).