Extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus:: insights from a metabolomics analysis
Extreme anoxia tolerance in embryos of the annual killifish Austrofundulus limnaeus:: insights from a metabolomics analysis
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DOI：
10.1242/jeb.005116
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发表时间：
2007-07-01
影响因子：
2.8
通讯作者：
Somero, George N.
中科院分区：
文献类型：
--
作者：
Podrabsky, Jason E.;Lopez, James P.;Somero, George N.
一年生鳉鱼Austrofundulus limnaeus生存在短暂的池塘栖息地生产耐旱滞育胚胎。这些胚胎可能经历缺氧作为其正常发育环境的一部分。我们对A. limnaeus胚胎在整个胚胎发育过程中。胚胎在早期发育过程中对缺氧有很大的耐受性，在滞育II期间达到顶峰。这种极端的耐缺氧性在滞育后II发育的前4天保留，然后丧失。缺氧期间的代谢似乎主要由乳酸盐的产生支持，丙氨酸和琥珀酸盐的产生贡献较小。缺氧胚胎也积累了大量的γ-氨基丁酸（GABA），一种潜在的神经功能保护剂。看来，该套件的字符与正常发展和进入滞育II在这个物种准备的胚胎长期生存在缺氧，即使胚胎暴露于有氧条件。这是第一次在脊椎动物胚胎中发现这种极端的耐缺氧现象，为脊椎动物耐缺氧研究提供了一个新的模型。
The annual killifish Austrofundulus limnaeus survives in ephemeral pond habitats by producing drought-tolerant diapausing embryos. These embryos probably experience oxygen deprivation as part of their normal developmental environment. We assessed the anoxia tolerance of A. limnaeus embryos across the duration of embryonic development. Embryos develop a substantial tolerance to anoxia during early development, which peaks during diapause II. This extreme tolerance of anoxia is retained during the first 4 days of post-diapause II development and is then lost. Metabolism during anoxia appears to be supported mainly by production of lactate, with alanine and succinate production contributing to a lesser degree. Anoxic embryos also accumulate large quantities of gamma-aminobutyrate ( GABA), a potential protector of neural function. It appears that the suite of characters associated with normal development and entry into diapause II in this species prepares the embryos for long-term survival in anoxia even while the embryos are exposed to aerobic conditions. This is the first report of such extreme anoxia tolerance in a vertebrate embryo, and introduces a new model for the study of anoxia tolerance in vertebrates.