Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging.
Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging.
复制标题
通过实时荧光成像同时检测四个细胞周期阶段。
DOI：
10.1007/978-1-0716-1258-3_3
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Lin,MichaelZ
中科院分区：
文献类型：
--
作者：
Bajar,BryceT;Lin,MichaelZ
通过细胞周期的可视化进展为研究增殖性疾病（如癌症）的发育、组织维持和生长失调提供了有价值的信息。荧光生物传感器的发展促进了分子过程的动态跟踪，包括细胞周期。基因编码的荧光指示剂组Fucci4使每个细胞周期阶段之间的转换可视化。在这里，我们描述了一种方法来跟踪进展，通过每个细胞周期阶段使用Fucci4在活的落射荧光成像。原则上，这种方法可以适用于任何四种光谱可分辨荧光指示剂的体外延时成像。
Visualizing progression through the cell cycle provides valuable information for the study of development, tissue maintenance, and dysregulated growth in proliferative diseases, such as cancer. Developments in fluorescent biosensors have facilitated dynamic tracking of molecular processes, including the cell cycle. The genetically encoded set of fluorescent indicators, Fucci4, enables the visualization of transitions between each cell cycle phase. Here, we describe a method to track progression through each cell cycle phase using Fucci4 in live epifluorescence imaging. In principle, this approach can be adapted to in vitro time-lapse imaging of any four spectrally resolvable fluorescent indicators.