LARGE INVERTED DUPLICATIONS ARE ASSOCIATED WITH GENE AMPLIFICATION
LARGE INVERTED DUPLICATIONS ARE ASSOCIATED WITH GENE AMPLIFICATION
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DOI：
10.1016/0092-8674(86)90328-4
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发表时间：
1986-05-09
期刊：
影响因子：
64.5
通讯作者：
FRIED, M
中科院分区：
文献类型：
--
作者：
FORD, M;FRIED, M
扩增的DNA可以在大重复单位的阵列中找到，每个重复单位包含一个标记基因和周围的DNA序列。对已建立的细胞系的扩增DNA序列进行评估，以确定反向复制形式的重复单位的存在。通过变性和快速复性后对S1水解性的不依赖于浓度的抗性，可以检测到反向复制DNA。利用该方法，在四个人类肿瘤细胞系中检测到含有myc基因的扩增DNA(16-50个拷贝/细胞)和在三个PALA耐药的BHK细胞系中检测到含有CAD基因的扩增DNA(30-2000个拷贝/细胞)的反向复制。反向复制与扩增DNA的广泛联系必然与扩增机制有关。
Amplified DNA can be found in arrays of large repeated units, with each repeat unit containing a marker gene and surrounding DNA sequences. Amplified DNA sequences from established cell lines were assessed for the presence of repeat units in the form of inverted duplications. Inverted duplicated DNA was detected by virtue of its concentration-independent resistance to S1 hydrolysis after denaturation and rapid renaturation. Using this assay, inverted duplications were detected in amplified DNA (both DM and HSR configurations) containing the myc gene (16-50 copies/cell) in four human tumor cell lines and in amplified DNA containing the CAD gene (30-2000 copies/cell) in three PALA-resistant BHK cell lines. The widespread association of inverted duplications with amplified DNA must bear on the amplification mechanism.