MOLECULAR CHARACTERIZATION AND EXPRESSION OF THE GENE ENCODING HUMAN ERYTHROID-POTENTIATING ACTIVITY
MOLECULAR CHARACTERIZATION AND EXPRESSION OF THE GENE ENCODING HUMAN ERYTHROID-POTENTIATING ACTIVITY
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DOI：
10.1038/315768a0
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发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
CLARK, SC
中科院分区：
文献类型：
--
作者：
GASSON, JC;GOLDE, DW;CLARK, SC
促红细胞生成素是红细胞生成的主要生理调节剂;然而，在体外研究中已经确定了另一类似乎在刺激红系祖细胞中很重要的介质。这些因子通常被称为爆发促进活性（BPA），因为它们刺激称为爆发形成单位-红系（BFU-E）的早期红系祖细胞的生长，产生多达数千个血红蛋白化细胞的集落1，2。我们最近报道了纯化的爆发促进活性的条件下的Mo T-淋巴母细胞细胞系感染的人T细胞嗜光性病毒II型（HTLV-II）3，4。这种相对分子质量（Mr）为28，000的纯化糖蛋白还通过更成熟的红细胞前体（CFU-E）刺激集落形成，因此被称为红细胞增强活性（EPA）5。纯化的EPA特异性刺激人和鼠红系细胞，而鼠白细胞介素-3（IL-3）刺激所有造血系的前体细胞6。我们在这里报告的分离的互补DNA分子克隆编码EPA和它的使用在生产EPA在COS（猴）细胞和CHO（中国仓鼠卵巢）细胞。我们还定义了EPA基因在人类DNA中的组织。
Erythropoietin is the primary physiological regulator of erythropoiesis; however,in vitrostudies have identified another class of mediators which appear to be important in stimulating erythroid progenitors. These factors have generally been referred to as burst-promoting activities (BPA), because they stimulate the growth of early erythroid progenitors referred to as burst-forming units-erythroid (BFU-E) which give rise to colonies of up to thousands of haemoglobinized cells1,2. We recently reported purification of a burst-promoting activity from medium conditioned by the Mo T-lymphoblast cell line infected with human T-cell lym-photropic virus type II (HTLV-II)3,4. This purified glycoprotein of relative molecular mass (Mr) 28,000 also stimulates colony formation by more mature erythroid precursors (CFU-E) and is therefore referred to as erythroid-potentiating activity (EPA)5. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages6. We report here the isolation of a complementary DNA molecular clone encoding EPA and its use in producing EPA in COS (monkey) cells and CHO (Chinese hamster ovary) cells. We also define the organization of the EPA gene in human DNA.