Effects of lysozyme on Alicyclobacillus acidoterrestris under laboratory conditions
Effects of lysozyme on Alicyclobacillus acidoterrestris under laboratory conditions
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DOI：
10.1111/ijfs.12302
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发表时间：
2014-01-01
影响因子：
3.3
通讯作者：
Corbo, Maria R.
中科院分区：
文献类型：
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作者：
Bevilacqua, Antonio;Ciuffreda, Emanuela;Corbo, Maria R.
本研究旨在探讨溶菌酶对嗜酸地酸芽孢杆菌的生物活性。测定溶菌酶（0.1 ~ 20ppm）对5种不同菌株的影响；采用微稀释法对孢子和营养细胞进行实验，以评估最小抑制浓度（MIC）。然后，通过传统的平板计数法评估两株菌株的孢子活力。最后，通过实验考察了溶菌酶对孢子萌发复杂现象的影响。溶菌酶对营养细胞的MIC为0.1 ~ 6ppm，对孢子的MIC为0.1 ~ 3ppm。关于孢子活力，溶菌酶的作用取决于其在肉汤中的量，因为在MIC时，它在24h后引起孢子的轻微减少（约1logcfuml(-1)）；否则，较高的浓度导致孢子水平低于检测极限。在孢子萌发方面，溶菌酶对这一现象有促进作用，使光密度降低66%。
This research was aimed at investigating the bioactivity of lysozyme towards Alicyclobacillus acidoterrestris. Lysozyme (0.1-20ppm) was tested towards five different strains; the experiments were performed on both spores and vegetative cells using a microdilution approach to assess the minimal inhibitory concentration (MIC). Thereafter, spore viability of two selected strains was evaluated through the traditional plate count. Finally, an experiment was run to assess the role of lysozyme on the complex phenomenon of spore germination. The MIC of lysozyme towards vegetative cells varied from 0.1 to 6ppm, while for the spores, it was from 0.1 to 3ppm. Concerning spore viability, the effect of lysozyme relied upon its amount on the broth, as at MIC it caused a slight reduction in spores (approximately 1logcfuml(-1)) after 24h; otherwise, higher concentrations caused a decrease in spore level below the detection limit. Concerning spore germination, lysozyme exerted a promoting effect on this phenomenon and reduced the optical density by 66%.