SPLICE-SITE SIGNALS OF MESSENGER-RNA PRECURSORS AS REVEALED BY COMPUTER-SEARCH - SITE-SPECIFIC MUTAGENESIS AND THALASSEMIA
SPLICE-SITE SIGNALS OF MESSENGER-RNA PRECURSORS AS REVEALED BY COMPUTER-SEARCH - SITE-SPECIFIC MUTAGENESIS AND THALASSEMIA
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DOI：
10.1093/oxfordjournals.jbchem.a135162
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
IIDA, Y
中科院分区：
文献类型：
--
作者：
IIDA, Y
许多真核基因被内含子中断，内含子通过RNA剪接机制从mRNA前体中移除。尽管剪接位点周围的核苷酸序列在各种基因之间具有相当大的同源性，但确切的剪接位点信号是未知的。以前，计算机搜索方法被应用于各种基因的一级核苷酸序列，以研究什么样的模式是识别5“-剪接位点（供体位点）的必要和充分序列。提出了4种常用模式，AG/GTA、/GTAAGT、RG/GTGAG和AG/GTXXGT，其中R = A或G，X = A、T、G或C，常用作这种信号。这篇论文检查了关于供体位点周围的位点特异性诱变和关于α-和β-珠蛋白基因地中海贫血。以上4种常见模式几乎可以解释所有这些结果。
Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are unknown. Previously, the computer searching method was applied to primary nucleotide sequences of various genes to study what kinds of patterns are the necessary and sufficient sequences for recognition of the 5''-splice site (donor site). It was proposed that 4 common patterns, AG/GTA, /GTAAGT, RG/GTGAG, and AG/GTXXGT, where R = A or G and X = A, T, G, or C, are often used as such signals. This paper examines a number of experimental results on site-specific mutagenesis around donor sites and on .alpha.- and .beta.-thalassemia in globin genes. The above 4 common patterns could explain almost all of those results.