Neoplastic transformation by the human gene N-myc.
Neoplastic transformation by the human gene N-myc.
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人类基因 N-myc 的肿瘤转化。
DOI：
10.1128/mcb.7.5.1638-1645.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
Bishop,JM
中科院分区：
文献类型：
--
作者：
Small,MB;Hay,N;Schwab,M;Bishop,JM
被称为N-mycare的基因的扩增和丰富表达经常在人类神经母细胞瘤的晚期发现，并可能在几种恶性人类肿瘤的发生中发挥作用。以前的研究表明，N-myccan与原癌基因c-Ha-ras的突变等位基因合作转化培养中的胚胎大鼠细胞。在这里，我们表明，N-myccan也可以单独行动，以引起肿瘤生长的一个既定的线大鼠成纤维细胞（大鼠-1）。我们使用重组DNA载体表达N-mycor及其同源基因c-mycin转染细胞。这两个基因引起的形态转化，锚定独立的增长，和致瘤性。我们注意到两个变量，似乎影响的能力，分离转化细胞的N-myc和c-myc：丰度的基因表达和生物选择，以消除未转化的细胞从文化。我们的研究结果支持了N-mycis是一个真实的原癌基因的信念，为N-mycis在人类肿瘤发生中的作用提供了进一步的可信度，并建立了一种方便的检测方法，可用于进一步探索N-mycis和c-myc的特性。
Amplification and abundant expression of a gene known as N-mycare found frequently in advanced stages of human neuroblastoma and may play a role in the genesis of several malignant human tumors. Previous studies have shown that N-myccan cooperate with a mutant allele of the proto-oncogene c-Ha-ras to transform embryonic rat cells in culture. Here we show that N-myccan also act alone to elicit neoplastic growth of an established line of rat fibroblasts (Rat-1). We used recombinant DNA vectors to express either N-mycor its kindred gene c-mycin transfected cells. Both genes caused morphological transformation, anchorage-independent growth, and tumorigenicity. We noticed two variables that appeared to influence the ability to isolate cells transformed by N-mycand c-myc: the abundance in which the genes were expressed and biological selection to eliminate untransformed cells from the cultures. Our findings sustain the belief that N-mycis an authentic proto-oncogene, lend further credibility to the role of N-mycin the genesis of human tumors, and establish a convenient assay that can be used to explore further the properties of both N-mycand c-myc.