Identification of the same polyomavirus species in different African horseshoe bat species is indicative of short-range host-switching events
Identification of the same polyomavirus species in different African horseshoe bat species is indicative of short-range host-switching events
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DOI：
10.1099/jgv.0.000935
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发表时间：
2017-11-01
影响因子：
3.8
通讯作者：
Sawa, Hirofumi
中科院分区：
文献类型：
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作者：
Carr, Michael;Gonzalez, Gabriel;Sawa, Hirofumi
多瘤病毒（PyV）被认为是高度宿主特异性在不同的哺乳动物物种，没有得到充分支持的证据宿主转换事件。我们研究了菊头蝠（Rhinolophus spp.）中PyVs的物种多样性和宿主特异性，一个广泛分布且种类繁多的哺乳动物属。我们注释了六个PyV基因组，包括四个新的PyV物种，基于大T抗原（LTAg）编码区内的成对身份。系统发育比较揭示了两种高度相关的PyV物种，一种在α多瘤病毒属和β多瘤病毒属中，存在于不同的菊头蝠宿主物种中（布氏菊头蝠和R.模拟器），提示短距离主机切换事件。在不同的菊头蝠宿主物种中的两对菊头蝠PyVs在其各自的LTAg编码序列上彼此具有99.9%和88.8%的相同性，因此构成相同的病毒物种。为了证实蝙蝠宿主的物种鉴定，我们分析了线粒体cytb和一个大的核内含子数据集来自六个独立的和中性的蝙蝠类群的基因座的利益。贝叶斯估计最近的共同祖先的年龄表明，几乎相同和更远的PyV物种分别在现在之前大约9.1 E4（5E 3 -2.8E5）和9.9E6（4 E6 - 18 E6）年分化，而蝙蝠宿主物种的分化时间为12.4E6（10.4E6-15.4E6）。我们的研究结果提供的证据表明，短距离的主机切换PyVs是可能的马蹄蝙蝠，这表明PyV的密切相关的哺乳动物物种之间的传输可以发生。
Polyomaviruses (PyVs) are considered to be highly host-specific in different mammalian species, with no well-supported evidence for host-switching events. We examined the species diversity and host specificity of PyVs in horseshoe bats (Rhinolophus spp.), a broadly distributed and highly speciose mammalian genus. We annotated six PyV genomes, comprising four new PyV species, based on pairwise identity within the large T antigen (LTAg) coding region. Phylogenetic comparisons revealed two instances of highly related PyV species, one in each of the Alphapolyomavirus and Betapolyomavirus genera, present in different horseshoe bat host species (Rhinolophus blasii and R. simulator), suggestive of short-range host-switching events. The two pairs of Rhinolophus PyVs in different horseshoe bat host species were 99.9 and 88.8% identical with each other over their respective LTAg coding sequences and thus constitute the same virus species. To corroborate the species identification of the bat hosts, we analysed mitochondrial cytb and a large nuclear intron dataset derived from six independent and neutrally evolving loci for bat taxa of interest. Bayesian estimates of the ages of the most recent common ancestors suggested that the near-identical and more distantly related PyV species diverged approximately 9.1 E4 (5E3-2.8E5) and 9.9E6 (4E6-18E6) years before the present, respectively, in contrast to the divergence times of the bat host species: 12.4E6 (10.4E6-15.4E6). Our findings provide evidence that short-range host-switching of PyVs is possible in horseshoe bats, suggesting that PyV transmission between closely related mammalian species can occur.