Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
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DOI：
10.3390/genes9120590
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发表时间：
2018-11
期刊：
影响因子：
3.5
通讯作者：
Kaida Xu;Hanxiang Xu;Zhiqiang Han
中科院分区：
文献类型：
--
作者：
Kaida Xu;Hanxiang Xu;Zhiqiang Han
热休克蛋白 70 (Hsp70) 是关键细胞过程和对环境变化的反应所必需的，然而，大黄鱼 (Larimichthys crocea) 中的 hsp70 基因家族成员数量未知。本研究通过大黄鱼基因组鉴定出17个hsp70基因。根据系统发育树，这些基因被分为七个进化上不同的组。在人类和斑马鱼中发现了这些 hsp70 基因的直系同源物。通过转录组数据研究冷热胁迫下大黄鱼hsp70基因家族的表达模式。冷或热应激后，17 个基因中有 6 个显着不受调节或下调。冷处理后肝脏中有2个基因显着上调，2个基因显着下调，而热处理后有5个基因显着上调，没有基因显着下调。检测到三种表达模式：严格热诱导的 hsp70、组成型表达和中度热诱导的 hsp70、以及组成型表达和较少胁迫依赖性的 hsp70 基因。所有这些发现将有助于更好地了解 hsp70 在抵御热挑战方面的生物学功能。
Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome of the large yellow croaker. These genes are divided into seven evolutionarily distinct groups according to a phylogenetic tree. The orthologs of these hsp70 genes were found in humans and zebrafish. The expression patterns of the hsp70 gene family in the large yellow croaker under cold and heat stress were studied by examining transcriptome data. Six out of 17 genes were significantly unregulated or downregulated after cold or heat stress. There were two genes significantly upregulated and two genes downregulated in the liver after cold treatment, while after heat treatment, five genes were significantly upregulated, and no genes were significantly downregulated. Three expression patterns were detected: strictly heat-inducible hsp70, constitutively expressed and moderately heat-inducible hsp70, and constitutively expressed and less stress-dependent hsp70 genes. All the findings will contribute to a better understanding of the biological function of hsp70s in defending against thermal challenges.