Probing embryonic stem cell autocrine and paracrine signaling using microfluidics.
Probing embryonic stem cell autocrine and paracrine signaling using microfluidics.
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DOI：
10.1146/annurev-anchem-062011-143122
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Voldman J
中科院分区：
文献类型：
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作者：
Przybyla L;Voldman J
While stem cell fate is traditionally manipulated by exogenously altering the cells’ extracellular signaling environment, the endogenous autocrine and paracrine signals produced by the cells also contribute to their two essential processes: self-renewal and differentiation. Autocrine and/or paracrine signals are fundamental to both embryonic stem cell self-renewal and early embryonic development, but the nature and contributions of these signals are often difficult to fully define using conventional methods. Microfluidic techniques have been used to explore the effects of cell-secreted signals by controlling cell organization or providing precise control over the spatial and temporal cellular microenvironment. Here we review how such techniques have begun to be adapted for use with embryonic stem cells, and illustrate how many remaining questions in embryonic stem cell biology could be addressed using microfluidic technologies.
