Lack of prostaglandin F2 alpha metabolism by human ocular tissues.
Lack of prostaglandin F2 alpha metabolism by human ocular tissues.
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DOI：
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发表时间：
1990-07
影响因子：
4.4
通讯作者：
A. Cheng-Bennett;J. Poyer;R. Weinkam;D. F. Woodward
中科院分区：
文献类型：
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作者：
A. Cheng-Bennett;J. Poyer;R. Weinkam;D. F. Woodward
通过跟踪PGF 2 α的代谢命运，比较了人和兔眼组织降解前列腺素（PGs）的能力。与人角膜、虹膜/睫状体或巩膜孵育5.0小时后，在体外未观察到代谢，如[3 H]-PGF 2 α浓度未降低或随时间推移出现[3 H]-PGF 2 α代谢物所示。同样，在体外与这些不同的兔眼组织或虹膜/睫状体匀浆孵育5.0小时后，或在兔眼局部应用后的体内，未观察到PGF 2 α代谢。唯一可检测的放射性峰对应于PGF 2 α。因此，可以得出结论，人和兔眼组织都缺乏通常通过15-OH脱氢、ω-氧化和β-氧化使野牡丹素失活的酶。与眼部代谢相反，PGF 2 α在存在兔肺匀浆的情况下代谢：[3 H]-PGF 2 α以时间依赖性方式随着代谢产物的同时形成而降低。肺匀浆中的这种代谢转化是NADP+依赖性的，放射性代谢峰与13，14-二氢-15-酮-PGF 2 α具有相同的保留时间。
The ability of human and rabbit ocular tissues to degrade prostaglandins (PGs) was compared by following the metabolic fate of PGF2 alpha. No metabolism was observed in vitro after 5.0 hr incubation with human cornea, iris/ciliary body, or sclera, as indicated by the absence of a decrease in [3H]-PGF2 alpha concentration or the appearance of [3H]-PGF2 alpha metabolites with time. Similarly, no metabolism of PGF2 alpha was observed in vitro after 5.0 hr incubation with these various rabbit ocular tissues or iris/ciliary body homogenate, or in vivo after topical application to rabbit eyes. The only detectable radioactive peak corresponded to PGF2 alpha. Therefore, it is concluded that both human and rabbit ocular tissues lack the enzymes that typically deactivate prostaglandins by 15-OH dehydrogenation, omega-oxidation, and beta-oxidation. In contrast to ocular metabolism, PGF2 alpha was metabolized in the presence of rabbit lung homogenate: [3H]-PGF2 alpha decreased with the simultaneous formation of a metabolite in a time-dependent manner. This metabolic transformation in lung homogenate was NADP+ dependent, and the radioactive metabolic peak had the same retention time as 13,14-dihydro-15-keto-PGF2 alpha.