LncRNA PAPAS promotes hepatocellular carcinoma by interacting with miR-188-5p
LncRNA PAPAS promotes hepatocellular carcinoma by interacting with miR-188-5p
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DOI：
10.1002/jcb.28623
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发表时间：
2019-08-01
影响因子：
4
通讯作者：
Liu, Shaoling
中科院分区：
文献类型：
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作者：
Ma, Jincai;Qin, Chengyong;Liu, Shaoling
据观察，长链非编码RNA（lncRNA）PAPAS调节rRNA合成，但其在人类疾病中的作用尚不清楚。我们的研究旨在探讨 PAPAS 在肝细胞癌 (HCC) 中的作用。在本研究中，我们发现与健康人和肿瘤邻近健康组织的血浆相比，HCC 和肿瘤患者的血浆中 PAPAS 表达上调。 HCC患者肿瘤组织和血浆中PAPAS的表达水平呈显着正相关。 PAPAS 的血浆水平有效地区分 I 期患者与健康对照。 MicroRNA (miR)-188-5p 在 HCC 患者的肿瘤组织中比在肿瘤邻近的健康组织中表达下调，并且与肿瘤组织中的 PAPAS 呈负相关，但在邻近的健康组织中则不然。 PAPAS 和 miR-188-5p 相互下调。 PAPAS过表达促进HCC细胞增殖，而miR-188-5p过表达抑制HCC细胞增殖。救援实验表明，miR-34a 过表达减弱了 PAPAS 过表达的影响。然而，PAPAS过表达未能显着影响癌细胞的迁移和侵袭。因此，lncRNA PAPAS 通过与 miR-188-5p 相互作用促进 HCC。
It has been observed that long noncoding RNA (lncRNA) PAPAS regulates rRNA synthesis, but its role in human diseases is unclear. Our study was carried out to investigate the role of PAPAS in hepatocellular carcinoma (HCC). In the present study, we found that PAPAS was upregulated both in plasma from patients with HCC and tumors compared with plasma from healthy people and tumor-adjacent healthy tissues. Expression levels of PAPAS in tumor tissues and plasma of patients with HCC were significantly and positively correlated. Plasma levels of PAPAS effectively distinguished stage I patients from healthy controls. MicroRNA (miR)-188-5p was downregulated in tumor tissues than in tumor-adjacent healthy tissues of patients with HCC, and was inversely correlated with PAPAS in tumor tissues but not in adjacent healthy tissues. PAPAS and miR-188-5p downregulated each other. PAPAS overexpression promoted, while miR-188-5p overexpression inhibited the HCC cell proliferation. Rescue experiment showed that miR-34a overexpression attenuated the effects of PAPAS overexpression. However, PAPAS overexpression failed to affect significantly cancer cell migration and invasion. Therefore, lncRNA PAPAS promotes HCC by interacting with miR-188-5p.