BMP-2 liberated from biomimetic implant coatings induces and sustains direct ossification in an ectopic rat model
BMP-2 liberated from biomimetic implant coatings induces and sustains direct ossification in an ectopic rat model
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DOI：
10.1016/j.bone.2005.02.005
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发表时间：
2005-05-01
期刊：
影响因子：
4.1
通讯作者：
Hunziker, EB
中科院分区：
文献类型：
--
作者：
Liu, Y;de Groot, K;Hunziker, EB
导论.使用大鼠模型，我们评估了异位骨形成的动力学和组织形态计量学与含BMP-2的仿生植入涂层的关系。材料和方法。设置一个实验组和三个对照组：涂有磷酸钙和BMP-2的生物仿生共沉淀层的钛合金盘[每个盘1.7 μ g未涂覆的椎间盘（对照）;仅涂覆有磷酸钙层的椎间盘生物相容性（对照）;和涂覆有磷酸钙层的盘，所述磷酸钙层击打表面吸附的BMP-2 [0.98 μ g/盘（对照）]。将椎间盘（每组n = 6）植入大鼠皮下，在5周内每隔7天取出椎间盘进行动力学、组织形态计量学、形态学和组织化学分析。在骨形成蛋白-2组中，在植入后2周首次观察到成骨活性，此后持续不减弱直至监测期结束。每个椎间盘的每周净骨形成率在2周时为5.8 mm（3），在5周时为3.64 mm（3）。在这些连接处每个椎间盘形成的总骨体积分别为5.8 mm（3）和10.3 mm（3）。通过直接骨化机制形成的骨组织沉积在距离植入物表面最多340英寸处。仿生涂层逐渐降解，最初由异物巨细胞单独，然后也由破骨细胞。40%的涂层材料（因此推测为结合的BMP-2）在监测期结束时保留。因此，已经释放了60%的所并入的BMP-2。在这5周的交界处，没有骨组织与任何对照种植体相关。结合到仿生磷酸钙涂层中的BMP-2不仅能够在体内异位部位诱导骨形成，而且能够在低药理学水平下以非常高的效力诱导骨形成，并且能够在相当长的时间内维持这种活性。成骨活性的维持对于牙科和骨科植入物的骨整合具有重要的临床意义。(c)2005年爱思唯尔公司All rights reserved.
Introduction. Using a rat model, we evaluated the kinetics and histomorphometry of ectopic bone formation in association with biomimetic implant coatings containing BMP-2.Materials and methods. One experimental and three control groups were set up: titanium-alloy discs coated with a biomimetically co-precipitated layer of calcium phosphate and BMP-2 [1.7 mu g per disc (incorporated-BMP group)]; uncoated discs (control); discs biomimetically coated with a layer of calcium phosphate alone (control); and discs biomimetically coated with a layer of calcium phosphate beating superficially adsorbed BMP-2 [0.98 mu g per disc (control)]. Discs (n = 6 per group) were implanted subcutaneously in rats and retrieved at 7-day intervals over a period of 5 weeks for kinetic, histomorphometrical, morphological and histochemical analyses.Results. In the incorporated-BMP-2 group, osteogenic activity was first observed 2 weeks after implantation and thereafter continued unabated until the end of the monitoring period. The net weekly rates of bone formation per disc were 5.8 mm(3) at 2 weeks and 3.64 mm(3) at 5 weeks. The total volumes of bone formed per disc at these junctures were 5.8 mm(3) and 10.3 mm(3), respectively. Bone tissue, which was formed by a direct ossification mechanism, was deposited at distances of up to 340 Inn from the implant surfaces. The biomimetic coatings were degraded gradually, initially by foreign body giant cells alone and then also by osteoclasts. Forty percent of the coating material (and thus presumably of the incorporated BMP-2) remained at the end of the monitoring period. Hence, 60% of the incorporated BMP-2 had been released. At this 5-week juncture, no bone tissue was associated with any of the control implants.Conclusion. BMP-2 incorporated into biomimetic calcium phosphate coatings is capable not only of inducing bone formation at an ectopic site in vivo but also of doing so with a very high potency at a low pharmacological level, and of sustaining this activity for a considerable period of time. The sustainment of osteogenic activity is of great clinical importance for the osseointegration of dental and orthopedic implants. (c) 2005 Elsevier Inc. All rights reserved.