Ontogeny of gene expression of Kir channel subunits in the rat
Ontogeny of gene expression of Kir channel subunits in the rat
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DOI：
10.1006/mcne.1997.0655
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Karschin, A
中科院分区：
文献类型：
--
作者：
Karschin, C;Karschin, A
我们报告详细的基因表达的Kir2和Kir3内向整流钾离子通道亚家族内的所有亚基在发展中的大鼠。使用原位杂交，发病的表达和细胞分布的成绩单在胚胎和出生后的大鼠大脑以及外周组织进行了评价。从胚胎第13天（E13）开始，除了“前脑”Kir2.3亚基，这是缺席的身体和大脑，直到E21，所有的亚基出现不同的，主要是不重叠的表达模式。在个体发育过程中，CNS中的表达变得更加广泛，导致在成年大鼠中观察到的广泛重叠的mRNA模式。亚单位主要存在于发育中的大脑区域，在成人中也是阳性的。大多数亚基，特别是Kir3.2和Kir3.4，在个体发育过程中在不同的脑核团中瞬时表达。Kir转录本的出现通常与神经发生过程中的渐进和隐性阶段无关，而是对每个亚基和任何特定神经元组进行差异调节。这是第一次证明，几个亚基，最丰富的Kir2.2，存在于周围神经系统的早期，即，在背根神经节、感觉颅神经节和交感神经节。此外，在所有亚基中，Kir3.3在整个胚胎神经系统和整个身体中普遍表达。总之，个体发生Kir通道表达的分析有助于解释Kir通道（如缺陷的韦弗Kir3.2基因所例示的）在CNS中的增殖、分化和突触发生期间的重要性。
We report the detailed gene expression of all subunits within the Kir2 and Kir3 inwardly rectifying K+ channel subfamilies in the developing rat. Using in situ hybridization, onset of expression and cellular distribution of transcripts in embryonic and postnatal rat brains as well as in peripheral tissues is evaluated. Beginning at embryonic day 13 (E13), except "forebrain" Kir2.3 subunits which are absent from the body and brain until E21, all subunits appear with distinct and mainly nonoverlapping expression patterns. During ontogenic development, expression in the CNS becomes more widespread, leading to widely overlapping mRNA patterns as observed in the adult rat. Subunits are mainly found in regions of the developing brain that are also positive in the adult. Most subunits, in particular Kir3.2 and Kir3.4, are expressed transiently in distinct brain nuclei during ontogeny. Appearance of Kir transcripts is not generally related to the progressive and recessive phases during neurogenesis, but rather regulated differentially for each subunit and any specific group of neurons. It is demonstrated for the first time that several subunits, and most abundantly Kir2.2, are present early in the peripheral nervous system, i.e., in dorsal root-, sensory cranial-, and sympathetic ganglia. Also, of all subunits Kir3.3 is ubiquitously expressed in the entire embryonic nervous system and throughout the body. In summary, analysis of ontogenic Kir channel expression helps deciphering the importance of Kir channels (as exemplified for the defective weaver Kir3.2 gene) during proliferation, differentiation, and synaptogenesis in the CNS.