Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay
Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay
复制标题
DOI：
10.2144/000112703
复制
发表时间：
2008-03-01
期刊：
影响因子：
2.7
通讯作者：
Leyland-Jones, Brian
中科院分区：
文献类型：
--
作者：
Abramovitz, Mark;Ordanic-Kodani, Maja;Leyland-Jones, Brian
福尔马林固定石蜡包埋(FFPE)的乳腺肿瘤组织很容易获得，这是一个很大程度上尚未开发的、具有长期随访数据的临床样本分子图谱的巨大资源。我们优化了制备总RNA的条件和参数，该总RNA具有必要的质量，用于DASL(cDNA介导的退火、选择、延伸和连接)试验，该试验使用仅100 ng的输入RNA同时分析502个基因的表达。
Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result ill the preparation of total RNA that is of the necessary quality,for use ill the DASL (cDNA-mediated annealing, selection, extension, and ligation) assay ill which expression of 502 genes are analyzed simultaneously using as little as 100 ng of input RNA.