Tumorigenicity of human breast cancer is associated with loss of the Ca2+-activated chloride channel CLCA2.
Tumorigenicity of human breast cancer is associated with loss of the Ca2+-activated chloride channel CLCA2.
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发表时间：
1999-11
期刊：
影响因子：
11.2
通讯作者：
A. Gruber;B. Pauli
中科院分区：
文献类型：
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作者：
A. Gruber;B. Pauli
人钙激活氯离子通道-2（CLCA 2）在正常乳腺上皮中表达，但在不同进展阶段的乳腺肿瘤中不表达。非转化和转化乳腺上皮细胞系的北方分析显示，非转化细胞系MCF 10 A和非致瘤细胞系MDA-MB-453中有CLCA 2表达，而所有致瘤细胞系均为阴性（MDA-MB-231、MDA-MB-435、MDA-MB-468和MCF 7）。当稳定地重新引入CLCA 2阴性MDA-MB-231和MDA-MB-435细胞中时，CLCA 2表达降低体外基质胶侵袭并诱导s.c.和裸鼠中MDA-MB-231细胞的转移性肿瘤。我们的研究结果表明，CLCA 2可能作为一种肿瘤抑制剂在乳腺癌。
The human Ca2+-activated chloride channel-2 (CLCA2) is expressed in normal breast epithelium but not in breast tumors of different stages of progression. Northern analysis of nontransformed and transformed breast epithelial cell lines revealed CLCA2 expression in the nontransformed cell line MCF10A and the nontumorigenic cell line MDA-MB-453, whereas all tumorigenic cell lines were negative (MDA-MB-231, MDA-MB-435, MDA-MB-468, and MCF7). When stably reintroduced into CLCA2-negative MDA-MB-231 and MDA-MB-435 cells, CLCA2 expression reduced Matrigel invasion in vitro and inducibility of s.c. and metastatic tumors of MDA-MB-231 cells in nude mice. Our results suggest that CLCA2 may act as a tumor suppressor in breast cancer.