Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells
Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells
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DOI：
10.1117/1.2722733
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Georgakoudi, Irene
中科院分区：
文献类型：
--
作者：
Boutrus, Steven;Greiner, Cherry;Georgakoudi, Irene
最近引入的体内流式细胞仪的实时，非侵入性检测和定量的细胞循环在血管系统中的小动物提供了一个强大的工具，用于跟踪不同类型的细胞在疾病进展中的作用。我们描述了一个便携式版本的设备，它提供了能力：a）激发和检测荧光在两个不同的颜色同时，和B）进行数据分析，在真实的时间。这些进步显著提高了仪器的实用性，并提供了一种提高检测特异性的手段。作为例子，我们提出了循环绿色荧光蛋白（GFP）标记的乳腺癌细胞在小鼠的血管系统中的消耗动力学，并在体内用两种抗体标记的循环造血干细胞的特异性检测。(C)2007年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
The recent introduction of the in vivo flow cytometer for real-time, noninvasive detection and quantification of cells circulating in the vasculature of small animals has provided a powerful tool for tracking the roles of different types of cells in disease progression. We describe a portable version of the device, which provides the capability to: a) excite and detect fluorescence at two distinct colors simultaneously, and b) perform data analysis in real time. These advances improve significantly the utility of the instrument and provide a means of increasing detection specificity. As examples, we present the depletion kinetics of circulating green fluorescent protein (GFP) labeled breast cancer cells in the vasculature of mice, and the specific detection of circulating hematopoietic stem cells labeled in vivo with two antibodies. (C) 2007 Society of Photo-Optical Instrumentation Engineers.