Three-Dimensional H-Scan Ultrasound Imaging of Early Breast Cancer Response to Neoadjuvant Therapy in a Murine Model.
Three-Dimensional H-Scan Ultrasound Imaging of Early Breast Cancer Response to Neoadjuvant Therapy in a Murine Model.
复制标题
小鼠模型早期乳腺癌对新辅助治疗反应的三维h扫描超声成像。
DOI：
10.1097/rli.0000000000000831
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发表时间：
2022-04-01
影响因子：
6.7
通讯作者：
Hoyt K
中科院分区：
文献类型：
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作者：
Tai H;Song J;Li J;Reddy S;Khairalseed M;Hoyt K
Three-dimensional (3-D) H-scan is a new ultrasound (US) technique that images the relative size of acoustic scatterers. The goal of this research was to evaluate use of 3-D H-scan ultrasound imaging for monitoring early breast cancer response to neoadjuvant therapy using a preclinical murine model of breast cancer. Preclinical studies were conducted using luciferase-positive breast cancer-bearing mice (N = 40). Anesthetized animals underwent ultrasound imaging at baseline before administration with an apoptosis-inducing drug or a saline control. Image data was acquired using an ultrasound scanner equipped with a volumetric transducer following either a shorter or longer term protocol. The later included bioluminescent imaging to quantify tumor cell viability. At termination, tumors were excised for ex vivo analysis. In vivo results showed that 3-D H-scan ultrasound imaging is considerably more sensitive to tumor changes after apoptosis-inducing drug therapy as compared to traditional B-scan ultrasound. While there was no difference at baseline (P > 0.99), H-scan ultrasound results from treated tumors exhibited progressive decreases in image intensity (up to 62.2% by day 3) that had a significant linear correlation with cancer cell nuclear size (R2 > 0.51, P < 0.001). Results were validated by histological data and a secondary longitudinal study with survival as the primary endpoint. Experimental results demonstrate that noninvasive 3-D H-scan ultrasound imaging can detect an early breast tumor response to apoptosis-inducing drug therapy. Local in vivo H-scan ultrasound image intensity correlated with cancer cell nuclear size, which is one of the first observable changes of a cancer cell undergoing apoptosis and confirmed using histological techniques. Early imaging results appear to provide prognostic insight on longer-term tumor response. Overall, 3-D H-scan ultrasound imaging is a promising technique that visualizes the entire tumor and detects breast cancer response at an early stage of therapy.
DOI：
10.7863/jum.2012.31.11.1759
发表时间：
2012-11
期刊：
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
影响因子：
--
作者：
Hoyt K;Sorace A;Saini R
通讯作者：
Saini R