Proteomic effects of repeated-dose oral exposure to 2-monochloropropanediol and its dipalmitate in rat testes
Proteomic effects of repeated-dose oral exposure to 2-monochloropropanediol and its dipalmitate in rat testes
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DOI：
10.1016/j.fct.2018.04.055
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发表时间：
2018-06-01
影响因子：
4.3
通讯作者：
Braeuning, Albert
中科院分区：
文献类型：
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作者：
Frenzel, Falko;Oberemm, Axel;Braeuning, Albert
2-和3-一氯丙二醇（2-MCPD）及其脂肪酸酯是食品污染物，在对含有脂肪和盐的食品进行热处理时同时形成。因此，例如，在速食食品或婴儿配方奶粉以及谷物或糕点中的精炼植物油中就会接触到 2- 或 3-MCPD。 2-MCPD 毒性的分子机制尚不清楚。在这里，我们对每天口服 10 mg/kg 体重的 2-MCPD 或等摩尔剂量的 2-MCPD 二棕榈酸酯作为代表性 2-MCPD 脂肪酸酯后，对 2-MCDP 诱导的睾丸变化进行了全面的蛋白质组学分析。在没有明显的组织病理学可检测毒性的情况下，记录了细胞蛋白质组特征的中度改变。这些观察结果符合 2-MCPD 和 3-MCPD 毒性的分子机制不同的假设。观察到的蛋白质组变化表明 2-MCPD 对丝裂原依赖性信号传导和线粒体能量利用的影响。首次提供的数据提供了对 2-MCPD 在睾丸组织中蛋白质组学效应的深入了解。
2- and 3-monochloropropanediol (2-MCPD) and their fatty acid esters are food contaminants which are concomitantly formed upon thermal treatment of foodstuff containing fats and salt. Exposure to 2- or 3-MCPD thus results, for example, from refined vegetable oils, in instant meals or infant formula, as well as in cereals or pastries. The molecular mechanisms of 2-MCPD toxicity are poorly understood. Here, we performed a comprehensive proteomic analysis of 2-MCDP-induced alterations in the testes from rats following oral administration of 10 mg/kg body weight per day 2-MCPD, or an equimolar dose of 2-MCPD dipalmitate as a representative 2-MCPD fatty acid ester. In the absence of overt histopathologically detectable toxicity, moderate alterations in cellular proteomic signatures were recorded. The observations are in line with the assumption that the molecular mechanisms of 2-MCPD and 3-MCPD toxicity differ. Observed proteomic alterations point towards effects of 2-MCPD on mitogen-dependent signaling and mitochondrial energy utilization. Presented data for the first time provide insight into proteomic effects of 2-MCPD in testicular tissue.