Multiple loci and genetic interactions involving flowering time genes regulate stem branching among natural variants of Arabidopsis
Multiple loci and genetic interactions involving flowering time genes regulate stem branching among natural variants of Arabidopsis
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DOI：
10.1111/nph.12306
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发表时间：
2013-08-01
期刊：
影响因子：
9.4
通讯作者：
Koornneef, Maarten
中科院分区：
文献类型：
--
作者：
Huang, Xueqing;Ding, Jia;Koornneef, Maarten
枝梢分枝是植物构型的主要决定因素。拟南芥茎叶腋分枝减少的遗传变异在一些自然材料中发现，在多亲本杂交后代中也存在较低的频率，利用与亲本回交获得的分离群体进行详细的遗传分析，并利用混合分离群体分析确定了控制茎分枝减少的等位变异。鉴定了对减少的茎分枝的自然变异有贡献的（减少的茎分枝1-8（RSB 1 -8））。遗传分析表明，RSB 6和RSB 7分别对应于开花时间基因FLOWERLOCUS C（FLC）和FRIGIDA（FRI），上位性调控茎分枝。此外，通过精细定位、转基因互补和表达分析证明，对应于RSB 8的FLOW LOCUS T（FT）不仅对开花时间产生多效性效应，而且在活性FRI和FLC等位基因的特定背景下，RSB性状也是如此。等位基因变异的后果仅在晚期表达-开花基因型揭示了新的和迄今为止未被怀疑的几个基因的作用，以及其在开花时间控制中的作用。
Shoot branching is a major determinant of plant architecture. Genetic variants for reduced stem branching in the axils of cauline leaves of Arabidopsis were found in some natural accessions and also at low frequency in the progeny of multiparent crosses.Detailed genetic analysis using segregating populations derived from backcrosses with the parental lines and bulked segregant analysis was used to identify the allelic variation controlling reduced stem branching.Eight quantitative trait loci (QTLs) contributing to natural variation for reduced stem branching were identified (REDUCED STEM BRANCHING 1-8 (RSB1-8)). Genetic analysis showed that RSB6 and RSB7, corresponding to flowering time genes FLOWERING LOCUS C (FLC) and FRIGIDA (FRI), epistatically regulate stem branching. Furthermore, FLOWERING LOCUS T (FT), which corresponds to RSB8 as demonstrated by fine-mapping, transgenic complementation and expression analysis, caused pleiotropic effects not only on flowering time, but, in the specific background of active FRI and FLC alleles, also on the RSB trait.The consequence of allelic variation only expressed in late-flowering genotypes revealed novel and thus far unsuspected roles of several genes well characterized for their roles in flowering time control.