IL-7 is expressed and secreted by human skeletal muscle cells
IL-7 is expressed and secreted by human skeletal muscle cells
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DOI：
10.1152/ajpcell.00094.2009
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发表时间：
2010-04-01
影响因子：
5.5
通讯作者：
Drevon, Christian A.
中科院分区：
文献类型：
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作者：
Haugen, Fred;Norheim, Frode;Drevon, Christian A.
Haugen F，Norheim F，Lian H，Wensaas AJ，Dueland S，贝格O，Funderud A，Skalhegg BS，Raastad T，Drevon CA. IL-7由人骨骼肌细胞表达和分泌。美国生理学杂志细胞生理学298：C807-C816，2010年。首次发表于2010年1月20日; doi：10.1152/ajpcell.00094.2009。除了产生运动之外，骨骼肌还可以具有作为分泌器官的功能。本研究的目的是鉴定骨骼肌细胞分泌的具有信号传导能力的新蛋白。IL-7在由卫星细胞分化的人肌管的原代培养物的条件培养基中检测到，并且浓度随着孵育时间而增加。通过免疫印迹和实时RT-PCR证实IL-7在蛋白和mRNA水平上的表达。此外，免疫荧光和特异性抗血清，多核肌管被发现共表达IL-7和肌球蛋白重链。在人肌管从卫星细胞分化的过程中，IL-7的表达在mRNA和蛋白水平上增加。相比之下，IL-7受体的mRNA表达在肌管中比卫星细胞低80%。在分化条件下与重组IL-7孵育导致末端肌球蛋白重链2（MYH 2）和肌细胞生成素（MYOG）的mRNA减少约35%，表明IL-7可能作用于卫星细胞，抑制肌纤维表型的发育。研究了细胞发育的替代途径，IL-7在Transwell系统中48小时后使卫星细胞的迁移增加了40%，而细胞增殖保持不变。在体内，实时RT-PCR分析股外侧肌（n = 10）和股外侧肌（n = 7）活组织检查从男性个体进行力量训练计划表明，11周后，平均IL-7 mRNA增加了三倍（P = 0.01）和四倍（P = 0.04），分别。总之，我们已经证明，IL-7是一种新的肌细胞因子调节在体外和体内，它可能在肌肉细胞发育的调节中发挥作用。
Haugen F, Norheim F, Lian H, Wensaas AJ, Dueland S, Berg O, Funderud A, Skalhegg BS, Raastad T, Drevon CA. IL-7 is expressed and secreted by human skeletal muscle cells. Am J Physiol Cell Physiol 298: C807-C816, 2010. First published January 20, 2010; doi:10.1152/ajpcell.00094.2009.-In addition to generating movement, skeletal muscle may have a function as a secretory organ. The aim of the present study was to identify novel proteins with signaling capabilities secreted from skeletal muscle cells. IL-7 was detected in media conditioned by primary cultures of human myotubes differentiated from satellite cells, and concentrations increased with incubation time. By immunoblotting and real-time RT-PCR IL-7 expression was confirmed at both protein and mRNA levels. Furthermore, with immunofluorescence and specific antisera, multinucleated myotubes were found to coexpress IL-7 and myosin heavy chain. During differentiation of human myotubes from satellite cells, IL-7 expression increased at mRNA and protein levels. In contrast, mRNA expression of the IL-7 receptor was 80% lower in myotubes compared with satellite cells. Incubations with recombinant IL-7 under differentiation conditions caused similar to 35% reduction in mRNA for the terminal myogenic markers myosin heavy chain 2 (MYH2) and myogenin (MYOG), suggesting that IL-7 may act on satellite cells to inhibit development of the muscle fiber phenotype. Alternative routes of cell development were investigated, and IL-7 increased migration of satellite cells by 40% after 48 h in a Transwell system, whereas cell proliferation remained unchanged. In vivo, real-time RT-PCR analysis of musculus vastus lateralis (n = 10) and musculus trapezius (n = 7) biopsies taken from male individuals undergoing a strength training program demonstrated that after 11 wk mean IL-7 mRNA increased by threefold (P = 0.01) and fourfold (P = 0.04), respectively. In conclusion, we have demonstrated that IL-7 is a novel myokine regulated both in vitro and in vivo, and it may play a role in the regulation of muscle cell development.