POTASSIUM AND RECOVERY OF ARTERIAL SMOOTH MUSCLE AFTER COLD STORAGE
POTASSIUM AND RECOVERY OF ARTERIAL SMOOTH MUSCLE AFTER COLD STORAGE
复制标题
DOI：
10.1085/jgp.46.1.19
复制
发表时间：
1962-01-01
影响因子：
3.8
通讯作者：
HEADINGS, VE
中科院分区：
文献类型：
--
作者：
BARR, L;BOHR, DF;HEADINGS, VE
通过观察在细胞内钾离子浓度[Ki]大小和[Ki][Ko]比值呈相反方向变化的条件下血管平滑肌的力学性能，研究了钾（K）对血管平滑肌性能的影响。在4℃长时间储存期间，动脉条失去钾离子以及对刺激作出反应的能力。随后将它们转移至38℃不同[Ko]的恢复溶液中。在较高[Ko]的溶液中，Ki重新积累的初始速率和稳态[Ki]更大。相反，在恢复过程中的任何时间，[Ko]越大，[Ki]:[Ko]比值越小。当动脉条置于温热的恢复溶液中时，它首先收缩然后舒张。初始收缩与恢复溶液的[Ko]无关，但随后的舒张速率和幅度随着[Ko]的增大而增大。随着肌肉进一步恢复，它们进入强直性收缩状态。恢复溶液中的[Ko]越高，这些收缩在更短的恢复时间后发生，并且以更快的速度达到更大的幅度。溶液转换实验表明，对电击的反应既取决于[Ki]:[Ko]比值，也取决于[Ki]。得出的结论是：（a）[Ki]增加会提高收缩性；（b）[Ki]增加会提高舒张速率；（c）[Ki]:[Ko]比值过高或过低都会降低兴奋性；（d）强直性缩短的程度取决于[Ki]:[Ko]比值。
The influence of K on the performance of vascular smooth muscle was studied by observing the mechanical performance of the muscle under conditions in which the magnitudes of [Ki] and of the [Ki][Ko] ratio varied in opposite directions. During prolonged storage at 4[degree]C the artery strips lost K and their ability to respond to stimuli. Subsequently they were transferred to recovery solutions of various [Ko] at 38CC. The initial rate of Ki reaccumulation and steady state [Ki] were greater in solutions of higher [Ko]. Conversely for any time during recovery, the greater [Ko], the smaller the [Ki]:[Ko] ratio. When the strip was placed in the warm recovery solution it first contracted and then relaxed. The initial contraction was not relatable to [Ko] of the recovery solution but the subsequent relaxation was greater in rate and magnitude as [Ko] was greater. As the muscles recovered further they went into tonic contracture. As the [Ko] in the recovery solutions was greater these contractures occurred after shorter recovery times, and attained greater amplitude at a faster rate. Solution-switching experiments indicated a dependence of responses to electrical shocks on both the [Ki]:[Ko] ratio and [Ki]. Conclusions drawn were: (a) increased [Kj] increases contractility, (b) increased [Kj] increases the rate of relaxation, (c) excitability is decreased by too high or low a [Ki]:[Ko] ratio, and (d) the extent of tonic shortening depends on the [Ki]:[Ko] ratio.