Autofocusing based on wavelength dependence of diffraction in two-wavelength digital holographic microscopy.
Autofocusing based on wavelength dependence of diffraction in two-wavelength digital holographic microscopy.
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DOI：
10.1364/ol.37.001172
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发表时间：
2012-04
期刊：
影响因子：
3.6
通讯作者：
Peng Gao;Baoli Yao;R. Rupp;Junwei Min;Rongli Guo;Baiheng Ma;Juanjuan Zheng;M. Lei;Shaohui Yan-Shaohui-Ya
中科院分区：
文献类型：
--
作者：
Peng Gao;Baoli Yao;R. Rupp;Junwei Min;Rongli Guo;Baiheng Ma;Juanjuan Zheng;M. Lei;Shaohui Yan-Shaohui-Ya
提出了一种基于双波长数字全息显微镜(TWDHM)衍射过程波长依赖性的自动聚焦方法。TWDHM采用红光和绿光照明，产生的全息图由彩色CCD摄像机同时记录。由于衍射过程的波长依赖性，重建平面离像面越远，红光和绿光分布的差异就越大。因此，可以通过寻找红光和绿光在其幅度分布上的变化的最小值来确定像平面。通过仿真和实验验证了该方法的可行性。
An autofocusing method for two-wavelength digital holographic microscopy (TWDHM) based on the wavelength dependence of the diffraction process is proposed. Red and green lights are employed for the illumination of the TWDHM, and the generated holograms are recorded simultaneously by a color CCD camera. Due to the wavelength dependency of the diffraction process, the farther the reconstruction plane is from the image plane, the larger the difference is between the red and green light distributions. Thus, the image plane can be determined by finding the minimum of the variation between the red and green lights on their amplitude distributions. The feasibility of the proposed method is demonstrated by simulation and experiment.