ANGIOBLAST DIFFERENTIATION AND MORPHOGENESIS OF THE VASCULAR ENDOTHELIUM IN THE MOUSE EMBRYO
ANGIOBLAST DIFFERENTIATION AND MORPHOGENESIS OF THE VASCULAR ENDOTHELIUM IN THE MOUSE EMBRYO
复制标题
DOI：
10.1016/0012-1606(91)90316-u
复制
发表时间：
1991-11-01
影响因子：
2.7
通讯作者：
HEIMARK, R
中科院分区：
文献类型：
--
作者：
COFFIN, JD;HARRISON, J;HEIMARK, R
应用单叶班迪藻B_4同工凝集素（BSLB_4）和血管性血友病因子（VWF）多克隆抗血清，研究了小鼠胚胎着床后内皮细胞的起源及其组织成血管的能力。对BSLB 4染色的整个安装和切片的胚胎进行检查，发现内皮细胞染色强烈，突出显示大血管、毛细血管和许多单个细胞。背主动脉的形成是第一次明显的E7时，许多凝集素阳性细胞出现在近轴和侧板中胚层。当发育进行到E8时，BSLB 4阳性细胞组织成头尾方向的细胞系，最终成为主动脉。在E9时，BSLB 4染色胚胎的所有血管，包括背主动脉、体间动脉和内膜。直到E8才检测到VWF表达，此时BSLB 4/VWF双染色切片显示背侧髂动脉为第一个VWF阳性血管，而其他可见BSLB 4的内皮对VWF免疫染色保持阴性。到E12，许多其他血管变得VWF阳性，包括主动脉弓、体间动脉和主静脉。然而，许多成血管细胞和毛细血管保持VWF阴性，反映了在其他物种的成年人中已报道的内皮细胞中VWF的异质性表达。这里报告的组织化学数据支持早期鸟类研究的结论，显示不同的血管模式，在最初形成的血管从孤立的成血管细胞（血管发生），其次是最初的血管结构的延伸和组织（血管生成）。此外，我们的数据表明，内皮细胞来自与背主动脉相关的不同VWF阳性来源，以及与胚胎中其他血管相关的VWF阴性来源。
Bandeiraea simplicifoliaB4 isolectin (BSLB4) and polyclonal antisera against von Willebrand factor (VWF) were used to study the origin of endothelial cells and their organization into blood vessels in the postimplantation mouse embryo. Examination of BSLB4-stained whole mounted and sectioned embryos revealed intense staining of the endothelium, highlighting large vessels, capillaries, and many individual cells. Dorsal aorta formation was first obvious at E7 when many lectin-positive cells appeared in paraxial and lateral plate mesoderm. As development proceeded to E8, BSLB4-positive cells became organized into craniocaudal lines destined to become the aorta proper. At E9, BSLB4 stained all vessels of the embryo including the dorsal aorta, the intersomitic arteries, and the endocardium. VWF expression was not detected until E8 when BSLB4/VWF double-stained sections revealed the dorsal aortae as the first VWF-positive vessels, while other endothelium visible with BSLB4 remained negative for VWF immunostaining. By E12 many other vessels became VWF-positive, including the aortic arches, the intersomitic arteries, and the cardinal veins. However, many angioblasts and capillaries remained VWF-negative, reflecting the heterogeneous expression of VWF among endothelium that has been reported in adults of other species. The histochemical data reported here support the conclusions of earlier avian studies by showing distinct vascular patterns in the initial formation of vessels from isolated angioblasts (vasculogenesis), followed by the extension and organization of the initial vascular structures (angiogenesis). Moreover, our data suggest that the endothelium arises from distinct VWF-positive sources associated with the dorsal aorta, as well as VWF-negative sources associated with other vessels in the embryo.