SIFamide peptides modulate cardiac activity differently in two species of Cancer crab
SIFamide peptides modulate cardiac activity differently in two species of Cancer crab
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DOI：
10.1016/j.ygcen.2019.06.008
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发表时间：
2019-10-01
影响因子：
2.7
通讯作者：
Christie, Andrew E.
中科院分区：
文献类型：
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作者：
Dickinson, Patsy S.;Samuel, Heidi M.;Christie, Andrew E.
SIFamide是一个广泛保守的节肢动物肽家族，其特征在于C-末端基序-SIFamide。在十足类甲壳动物中，已知SIFamide的两种异构体，GYRKPPFNGSIFamide（Gly（1）-SIFamide），其在该目中几乎普遍保守，以及VYRKPPFNGSIFamide（瓦尔（1）-SIFamide），其仅从鲎属的成员中已知。虽然很多工作都集中在十足类动物中SIFamide亚型的鉴定上，但很少有直接的证据表明该肽家族成员的生理功能。在此，我们评估了Gly（1）-和瓦尔（1）-SIFamide对两种密切相关的巨蟹蟹（北巨蟹蟹和北巨蟹蟹）心脏神经肌肉系统的影响。在每个物种中，两种肽都具有心脏活性，在给定物种中两种亚型引起的剂量依赖性作用相同。生物活性的阈值浓度在通常与激素递送相关的范围内，即，10（-9）至10（-8）M。有趣的是，相当令人惊讶的是，虽然SIFamide对心输出量的预测作用在两种C。borealis和C.频率效应在C. borealis，而振幅效应在C.我是阿克罗塔斯这些发现表明，虽然SIFamide可能会增加两种螃蟹的心输出量，但实现这一目标的机制在两个物种中是不同的。免疫组织化学/质谱数据表明，SIFamide是通过尿道而不是局部递送到心脏的，激素释放的来源是两种癌症物种中的中肠上皮内分泌细胞。如果是这样的话，中肠衍生的SIFamide可能在消化过程中起到心输出量调节剂的作用。
The SIFamides are a broadly conserved arthropod peptide family characterized by the C-terminal motif-SIFamide. In decapod crustaceans, two isoforms of SIFamide are known, GYRKPPFNGSIFamide (Gly(1)-SIFamide), which is nearly ubiquitously conserved in the order, and VYRKPPFNGSIFamide (Val(1)-SIFamide), known only from members of the astacidean genus Homarus. While much work has focused on the identification of SIFamide isoforms in decapods, there are few direct demonstrations of physiological function for members of the peptide family in this taxon. Here, we assessed the effects of Gly(1)- and Val(1)-SIFamide on the cardiac neuromuscular system of two closely related species of Cancer crab, Cancer borealis and Cancer irroratus. In each species, both peptides were cardioactive, with identical, dose-dependent effects elicited by both isoforms in a given species. Threshold concentrations for bioactivity are in the range typically associated with hormonal delivery, i.e., 10(-9) to 10(-8) M. Interestingly, and quite surprisingly, while the predicted effects of SIFamide on cardiac output are similar in both C. borealis and C. irroratus, frequency effects predominate in C. borealis, while amplitude effects predominate in C. irroratus. These findings suggest that, while SIFamide is likely to increase cardiac output in both crabs, the mechanism through which this is achieved is different in the two species. Immunohistochemical/mass spectrometric data suggest that SIFamide is delivered to the heart hormonally rather than locally, with the source of hormonal release being midgut epithelial endocrine cells in both Cancer species. If so, midgut-derived SIFamide may function as a regulator of cardiac output during the process of digestion.