INVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACK
INVOLVEMENT OF GTP-BINDING G PROTEINS IN TRANSPORT THROUGH THE GOLGI STACK
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DOI：
10.1016/0092-8674(87)90591-5
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发表时间：
1987-12-24
期刊：
影响因子：
64.5
通讯作者：
ROTHMAN, JE
中科院分区：
文献类型：
--
作者：
MELANCON, P;GLICK, BS;ROTHMAN, JE
在无细胞系统中，GTP.Gamma.S不可逆转地抑制高尔基体堆叠的连续隔室之间的蛋白质运输。添加铝离子增强的氟化物也会引起强烈的抑制作用。这些都是鸟嘌呤核苷酸结合或调节的“G”蛋白参与的特征。GTP.Gamma.S的抑制需要一种胞质抑制因子，该因子在抑制过程中与高尔基体膜结合。预培养实验表明，GTPγS阻断了受体高尔基体膜的功能，但不能阻断供体高尔基体膜的功能。更具体地说，囊泡附着和实际融合事件之间的处理步骤似乎受到了影响。电子显微镜显示，在GTP-Gamma.S抑制期间，与高尔基体池相关的非笼络蛋白涂层的芽和囊泡相应地积累了5倍。
GTP.gamma.S irreversibly inhibits protein transport between successive compartments of the Golgi stack in a cell free system. Fluoride, potentiated by the addition of aluminum ion, also causes a strong inhibition. These are hallmarks of the involvement of a guanine nucleotide-binding or regulatory "G" protein. Inhibition by GTP.gamma.S requires a cytosolic inhibitory factor that binds to Golgi membranes during inhibition. Preincubation experiments reveal the GTP.gamma.S blocks the function of acceptor Golgi but not donor Golgi membranes. More specifically, a processing step in between vesicle attachment and the actual fusion event seems to be affected. Electron microscopy demonstrates a corresponding 5-fold accumulation of non-clathrin-coated buds and vesicles associated with the Golgi cisternae during inhibition by GTP.gamma.S.