Rapid adaptation of endocytosis, exocytosis, and eisosomes after an acute increase in membrane tension in yeast cells.
Rapid adaptation of endocytosis, exocytosis, and eisosomes after an acute increase in membrane tension in yeast cells.
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DOI：
10.7554/elife.62084
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发表时间：
2021-05-13
期刊：
影响因子：
7.7
通讯作者：
Berro, Julien
中科院分区：
文献类型：
--
作者：
Lemiere, Joel;Ren, Yuan;Berro, Julien
在真核生物网格蛋白介导的内吞作用（CME）中，肌动蛋白组装需要克服大的膜张力和膨压。然而，肌动蛋白机器适应不同的膜张力的分子机制仍然未知。此外，当细胞受到低渗休克的挑战时，它们是如何降低膜张力的仍然不清楚。我们使用定量显微镜来证明细胞使用三种平行机制快速降低其膜张力。除了利用它们的细胞壁进行机械保护外，酵母细胞在一分钟的时间尺度上分解Eisosome以缓冲膜张力的适度变化。同时，胞吞速率暂时降低2-6分钟，胞吐速率增加至少5分钟，使细胞向质膜添加大量膜。我们建立在这些结果提交细胞膜张力的突然增加，并确定内吞肌动蛋白机制的裂变酵母细胞迅速适应执行CME。我们的研究揭示了膜张力调节、内吞作用和胞吐作用之间的紧密联系。
During clathrin-mediated endocytosis (CME) in eukaryotes, actin assembly is required to overcome large membrane tension and turgor pressure. However, the molecular mechanisms by which the actin machinery adapts to varying membrane tension remain unknown. In addition, how cells reduce their membrane tension when they are challenged by hypotonic shocks remains unclear. We used quantitative microscopy to demonstrate that cells rapidly reduce their membrane tension using three parallel mechanisms. In addition to using their cell wall for mechanical protection, yeast cells disassemble eisosomes to buffer moderate changes in membrane tension on a minute time scale. Meanwhile, a temporary reduction in the rate of endocytosis for 2–6 min and an increase in the rate of exocytosis for at least 5 min allow cells to add large pools of membrane to the plasma membrane. We built on these results to submit the cells to abrupt increases in membrane tension and determine that the endocytic actin machinery of fission yeast cells rapidly adapts to perform CME. Our study sheds light on the tight connection between membrane tension regulation, endocytosis, and exocytosis.