Multifunctional quantum dot-polypeptide hybrid nanogel for targeted imaging and drug delivery.
Multifunctional quantum dot-polypeptide hybrid nanogel for targeted imaging and drug delivery.
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DOI：
10.1039/c4nr03058c
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发表时间：
2014-09
期刊：
影响因子：
6.7
通讯作者：
Jie Yang;Ming-Hao Yao;Lang Wen;Ji-tao Song;Mingzhen Zhang;Yuanyang Zhao;Bo Liu
中科院分区：
文献类型：
--
作者：
Jie Yang;Ming-Hao Yao;Lang Wen;Ji-tao Song;Mingzhen Zhang;Yuanyang Zhao;Bo Liu
通过金属亲和驱动人工多肽与CdSe-ZnS核壳量子点的自组装，制备了一种新型的具有靶向成像和药物释放特性的多功能量子点-多肽杂化纳米凝胶。通过毛细管电泳、透射电子显微镜和动态光散射测量，证实了量子点表面具有可调的类纳米结构，由两层疏水层和一层亲水层组成.疏水性和亲水性药物可以同时负载在QD-多肽纳米凝胶中。载药QD-多肽纳米凝胶的体外药物释放随温度、pH和竞争对手而强烈变化。通过共聚焦显微镜和流式细胞术分析，具有精氨酸-甘氨酸-天冬氨酸（RGD）基序的载药QD-多肽纳米凝胶在αvβ3过表达的HeLa细胞中表现出有效的受体介导的内吞作用，但在对照MCF-7细胞中没有。相比之下，非靶向QD-多肽纳米凝胶在HeLa细胞中显示出最小的结合和摄取。与原始量子点相比，量子点-多肽纳米凝胶显示出对HeLa细胞和NIH 3 T3细胞的体外细胞毒性降低。此外，靶向QD-多肽纳米凝胶对正常NIH 3 T3细胞的细胞毒性低于HeLa癌细胞。这些结果表明，在单个QD-多肽纳米凝胶中整合成像和药物递送功能具有在癌症诊断、成像和治疗中应用的潜力。
A new type of multifunctional quantum dot (QD)-polypeptide hybrid nanogel with targeted imaging and drug delivery properties has been developed by metal-affinity driven self-assembly between artificial polypeptides and CdSe-ZnS core-shell QDs. On the surface of QDs, a tunable sandwich-like microstructure consisting of two hydrophobic layers and one hydrophilic layer between them was verified by capillary electrophoresis, transmission electron microscopy, and dynamic light scattering measurements. Hydrophobic and hydrophilic drugs can be simultaneously loaded in a QD-polypeptide nanogel. In vitro drug release of drug-loaded QD-polypeptide nanogels varies strongly with temperature, pH, and competitors. A drug-loaded QD-polypeptide nanogel with an arginine-glycine-aspartic acid (RGD) motif exhibited efficient receptor-mediated endocytosis in αvβ3 overexpressing HeLa cells but not in the control MCF-7 cells as analyzed by confocal microscopy and flow cytometry. In contrast, non-targeted QD-polypeptide nanogels revealed minimal binding and uptake in HeLa cells. Compared with the original QDs, the QD-polypeptide nanogels showed lower in vitro cytotoxicity for both HeLa cells and NIH 3T3 cells. Furthermore, the cytotoxicity of the targeted QD-polypeptide nanogel was lower for normal NIH 3T3 cells than that for HeLa cancer cells. These results demonstrate that the integration of imaging and drug delivery functions in a single QD-polypeptide nanogel has the potential for application in cancer diagnosis, imaging, and therapy.