In situ molecular detection of some white-rot and brown-rot basidiomycetes infecting temperate and tropical woods
In situ molecular detection of some white-rot and brown-rot basidiomycetes infecting temperate and tropical woods
复制标题
感染温带和热带木材的一些白腐病和褐腐担子菌的原位分子检测
DOI：
10.1139/x05-056
复制
发表时间：
2005
影响因子：
2.2
通讯作者：
Y. Prin
中科院分区：
文献类型：
--
作者：
A. Zaremski;M. Ducousso;O. Domergue;Joël Fardoux;Cécile Rangin;D. Fouquet;H. Joly;Christian Sales;B. Dreyfus;Y. Prin
木材腐朽白腐和褐腐真菌对热带和温带的商业和制造木材具有重大的经济影响。本研究的目的是设计一种分子方法，再加上聚合酶链反应（PCR）和DNA测序，使早期鉴定各种形式的真菌腐烂的各种类型的木材。由此产生的工具可用于证明商业木材的健康状况，并更有效地利用化学品，从而减少其对环境的负面影响。根据EN 113标准方法，在维氏丝孔菌、Coniophora puteana、密粘褶菌、血红密孔菌和变色栓菌的存在下，体外孵育本氏双蕊木（Distemonanthus benthamianus）、森林山毛榉（Fagus sylvatica）、翼片杉（Lophira阿拉塔）、樟子松（Pinus sylvestris）和安哥拉密壳藻（Pycnanthus angolensis）的边材板。平均质量损失范围从2.6%到25.0%，表明所有的木材样品实际上已经被感染，使我们能够测试我们的方法的可靠性。PCR产物经…
Wood-decay white-rot and brown-rot fungi have a major economic impact on commercial and manufactured tropical and temperate woods. The aim of this study was to design a molecular method, coupled with polymerase chain reaction (PCR) and DNA sequencing, to enable early identification of various forms of fungal decay in various types of wood. The resulting tool could be used to certify the healthiness of commercial woods and also to make more efficient use of chemicals and thus reduce their negative environmental impact. Sapwood plates of Distemonanthus benthamianus,Fagus sylvatica, Lophira alata, Pinus sylvestris, and Pycnanthus angolensis were incubated in vitro in the presence of Fibroporia vaillantii, Coniophora puteana, Gloeophyllum trabeum, Pycnoporus sanguineus, and Trametes versicolor according to the EN 113 standard method. Average mass losses ranging from 2.6% to 25.0% indicated that all wood samples had been actually infected and enabled us to test the reliability of our method. PCR products were ...