Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing.
Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing.
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DOI：
10.1186/1471-2164-14-246
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发表时间：
2013-04-11
期刊：
影响因子：
4.4
通讯作者：
Archer DB
中科院分区：
文献类型：
--
作者：
Novodvorska M;Hayer K;Pullan ST;Wilson R;Blythe MJ;Stam H;Stratford M;Archer DB
进行全基因组分析以评估萌发A的转录格局。使用下一代RNA测序和基因芯片。还检查了分生孢子萌发期间的贮藏化合物的代谢，并将其与来自相关基因的转录物水平进行比较。休眠分生孢子的转录组被证明是高度分化的萌发分生孢子和响应于环境变化的主要变化发生在萌发的第一个小时内。休眠的打破与参与蛋白质生物合成、RNA周转和呼吸代谢的基因的转录水平增加有关。在发芽开始时参与硝酸盐代谢的基因的转录水平增加意味着其用作氮源。休眠分生孢子的转录组中含有在萌发过程中发生变化的反义转录物的重要组成部分。炭疽菌分生孢子含有参与发酵、孢子发生和乙醛酸循环的基因的转录本。休眠分生孢子中这种转录本的存在可能表明在饥饿诱导的分生孢子形成期间或在休眠期间用于维持目的的非碳水化合物底物产生能量。萌发开始后，内部存储化合物的代谢立即开始，以及相关基因的转录本的存在下，表明分生孢子引发萌发的开始。对于一些基因，反义转录在从休眠分生孢子到完全活跃的萌发体的过渡中受到调节。
Genome-wide analysis was performed to assess the transcriptional landscape of germinating A. niger conidia using both next generation RNA-sequencing and GeneChips. The metabolism of storage compounds during conidial germination was also examined and compared to the transcript levels from associated genes. The transcriptome of dormant conidia was shown to be highly differentiated from that of germinating conidia and major changes in response to environmental shift occurred within the first hour of germination. The breaking of dormancy was associated with increased transcript levels of genes involved in the biosynthesis of proteins, RNA turnover and respiratory metabolism. Increased transcript levels of genes involved in metabolism of nitrate at the onset of germination implies its use as a source of nitrogen. The transcriptome of dormant conidia contained a significant component of antisense transcripts that changed during germination. Dormant conidia contained transcripts of genes involved in fermentation, gluconeogenesis and the glyoxylate cycle. The presence of such transcripts in dormant conidia may indicate the generation of energy from non-carbohydrate substrates during starvation-induced conidiation or for maintenance purposes during dormancy. The immediate onset of metabolism of internal storage compounds after the onset of germination, and the presence of transcripts of relevant genes, suggest that conidia are primed for the onset of germination. For some genes, antisense transcription is regulated in the transition from resting conidia to fully active germinants.
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影响因子：
3.6
作者：
Hoffmann, B;Wanke, C;Braus, GH
通讯作者：
Braus, GH
影响因子：
4.8
作者：
Brengues, M;Pintard, L;Lapeyre, B
通讯作者：
Lapeyre, B
影响因子：
3
作者：
Ebel, Frank;Schwienbacher, Monika;Brock, Matthias
通讯作者：
Brock, Matthias
影响因子：
3.6
作者：
d'Enfert, C;Bonini, BM;Terenzi, HF
通讯作者：
Terenzi, HF
影响因子：
3.2
作者：
CHRISTENSEN, RL;SCHMIT, JC
通讯作者：
SCHMIT, JC