Extracellular stimulation window explained by a geometry-based model of the neuron-electrode contact
Extracellular stimulation window explained by a geometry-based model of the neuron-electrode contact
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DOI：
10.1109/tbme.2002.804504
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发表时间：
2002-12-01
影响因子：
4.6
通讯作者：
Marani, E
中科院分区：
文献类型：
--
作者：
Buitenweg, JR;Rutten, WLC;Marani, E
对完全密封微电极的单个培养神经元的细胞外刺激通常使用至少 200 nA 的阳极或双相电流进行。然而，最近获得的实验数据证明了使用振幅较小的阴极电流脉冲刺激神经元的可能性。此外，还观察到刺激窗口。这些发现可以通过有限元模型来解释，该模型允许对神经元-电极界面进行基于几何的电表示，并可用于详细探索细胞外刺激所需的条件。膜密封部分电压敏感通道的调节似乎是成功阴极刺激的关键。此外，刺激窗口的上限可以解释为神经元膜电生理学的正常结果。
Extracellular stimulation of single cultured neurons which are completely sealing a microelectrode is usually performed using anodic or biphasic currents of at least 200 nA. However, recently obtained experimental data demonstrate the possibility to stimulate a neuron using cathodic current pulses with less amplitude. Also, a stimulation window is observed. These findings can be explained by a finite-element model which permits geometry-based electrical representation of the neuron-electrode interface and can be used to explore the required conditions for extracellular stimulation in detail. Modulation of the voltage sensitive channels in the sealing part of the membrane appears to be the key to successful cathodic stimulation. Furthermore, the upper limit of the stimulation window can be explained as a normal consequence of the neuronal membrane electrophysiology.