The Arabidopsis GAGA-Binding Factor BASIC PENTACYSTEINE6 Recruits the POLYCOMB-REPRESSIVE COMPLEX1 Component LIKE HETEROCHROMATIN PROTEIN1 to GAGA DNA Motifs
The Arabidopsis GAGA-Binding Factor BASIC PENTACYSTEINE6 Recruits the POLYCOMB-REPRESSIVE COMPLEX1 Component LIKE HETEROCHROMATIN PROTEIN1 to GAGA DNA Motifs
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DOI：
10.1104/pp.15.00409
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发表时间：
2015-07-01
期刊：
影响因子：
7.4
通讯作者：
Wanke, Dierk
中科院分区：
文献类型：
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作者：
Hecker, Andreas;Brand, Luise H.;Wanke, Dierk
polycomb - suppression complexes （prc）通过抑制参与多种功能的大量基因在发育过程中发挥关键作用。然而，关于prc沉默机制以及它们针对特定基因组区域的靶向性，仍有许多有待发现。除其他机制外，动物体内的ga结合因子可以以序列特异性的方式引导PRC成员获得polycomb响应的DNA元件。在这里，我们发现拟南芥（拟南芥）ga -motif结合因子蛋白BASIC PENTACYSTEINE6 （BPC6）在体内与LIKE异染色质蛋白1 (LHP1) （PRC1成分）相互作用，并与VERNALIZATION2 (VRN2) （PRC2成分）相互作用。通过改进的DNA-蛋白相互作用酶联免疫吸附实验，我们可以证明BPC6是必需的，并且足以在体外将LHP1招募到含有GAGA基序的DNA探针中。我们还发现LHP1与VRN2相互作用，因此可以作为BPC6和VRN2之间可能的支架。LHP1 -4 - bpc4 - bpc6三突变体表现为多效型、极度矮化和早开花，揭示了LHP1与II类植物BPC成员的协同作用。转录组分析支持这种协同作用，并提出可能通过组蛋白H3赖氨酸-27三甲基化协同抑制同源基因的功能。因此，我们的研究结果表明，动物和植物的ga结合因子在募集PRC1和PRC2组分到polycomb响应的DNA元件类GAGA基序时具有惊人的相似性，这一定是通过趋同进化进化而来的。
Polycomb-repressive complexes (PRCs) play key roles in development by repressing a large number of genes involved in various functions. Much, however, remains to be discovered about PRC-silencing mechanisms as well as their targeting to specific genomic regions. Besides other mechanisms, GAGA-binding factors in animals can guide PRC members in a sequence-specific manner to Polycomb-responsive DNA elements. Here, we show that the Arabidopsis (Arabidopsis thaliana) GAGA-motif binding factor protein BASIC PENTACYSTEINE6 (BPC6) interacts with LIKE HETEROCHROMATIN PROTEIN1 (LHP1), a PRC1 component, and associates with VERNALIZATION2 (VRN2), a PRC2 component, in vivo. By using a modified DNA-protein interaction enzyme-linked immunosorbant assay, we could show that BPC6 was required and sufficient to recruit LHP1 to GAGA motif-containing DNA probes in vitro. We also found that LHP1 interacts with VRN2 and, therefore, can function as a possible scaffold between BPC6 and VRN2. The lhp1-4 bpc4 bpc6 triple mutant displayed a pleiotropic phenotype, extreme dwarfism and early flowering, which disclosed synergistic functions of LHP1 and group II plant BPC members. Transcriptome analyses supported this synergy and suggested a possible function in the concerted repression of homeotic genes, probably through histone H3 lysine-27 trimethylation. Hence, our findings suggest striking similarities between animal and plant GAGA-binding factors in the recruitment of PRC1 and PRC2 components to Polycomb-responsive DNA element-like GAGA motifs, which must have evolved through convergent evolution.