The influence of temperature on metabolic and cellular protection of the heart during long-term ischemia: A study using P-31 magnetic resonance spectroscopy and biochemical analyses'
The influence of temperature on metabolic and cellular protection of the heart during long-term ischemia: A study using P-31 magnetic resonance spectroscopy and biochemical analyses'
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DOI：
10.1006/cryo.1998.2126
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发表时间：
1998-12-01
期刊：
影响因子：
2.7
通讯作者：
Cozzone, PJ
中科院分区：
文献类型：
--
作者：
Bernard, M;Cartoux, C;Cozzone, PJ
我们比较了两种不同的冷温度（低于10摄氏度）的心肌缺血的影响，通过测量大量的各种血液动力学和代谢参数在缺血和再流。用我们实验室开发的保存溶液将分离的等容大鼠心脏停搏，然后在相同溶液中冷藏5小时（4 ℃，组I;和7.5 ℃，组II）。在15 ℃下再进行50分钟的局部缺血，同时进行间歇性心脏停搏液输注后，在37 ℃下对心脏再灌注60分钟。在控制期和再流期间评估功能。高能磷酸盐和细胞内pH值，其次是P-31磁共振波谱。通过生化测定和HPLC对冠状动脉流出物和冷冻心脏中的代谢物和酶进行分析，以评估细胞完整性。缺血时，能量池在4 ℃下保存较好（ATP在4 ℃缺血结束时，I组为59 +/- 7%，II组为31 +/- 5%，P < 0.01），（P < 0.05），但当温度升高到7.5 ℃时，膜保护作用更高（肌酸激酶渗漏减少，I组89 +/- 16 IU/min vs II组51 +/- 5 IU/min，P < 0.05）。结果，在7.5 ℃保存的心脏中，以速率压力乘积表示的功能恢复更高（在复流结束时，I组恢复52 +/- 6%，II组恢复77 +/- 7%，P < 0.05）。总之，在7.5摄氏度下冷藏比在4摄氏度下冷藏提供更好的保护。(C)北京：科学出版社.
We have compared the influence of two different cold temperatures (below 10 degrees C) for cardiac ischemia by measuring a large variety of hemodynamic and metabolic parameters during ischemia and reflow. Isolated isovolumic rat hearts were arrested with a preservation solution which was developed in our laboratory and then submitted to 5 h of cold storage (4 degrees C, group I; and 7.5 degrees C, group II) in the same solution. After an additional period of 50 min of ischemia at 15 degrees C with intermittent cardioplegic infusion, hearts were reperfused for 60 min at 37 degrees C. Function was assessed during the control period and reflow. High-energy phosphates and intracellular pH were followed by P-31 magnetic resonance spectroscopy. Analyses of metabolites and enzymes were performed by biochemical assays and HPLC in coronary effluents and in freeze-clamped hearts to assess cellular integrity. The energetic pool was better preserved at 4 degrees C during ischemia (ATP at the end of 4 degrees C ischemia, 59 +/- 7% in group I vs 31 +/- 5% in group II, P < 0.01) and reflow (P < 0.05) but membrane protection was higher when increasing the temperature to 7.5 degrees C (reduction of creatine kinase leakage, 89 +/- 16 IU/min in group I vs 51 +/- 5 IU/min in group II, P < 0.05). As a result, functional recovery, represented by the rate pressure sure product, was higher in hearts preserved at 7.5 degrees C (52 +/- 6% recovery in group I vs 77 +/- 7% in group II at the end of reflow, P < 0.05). Altogether, cold storage at 7.5 degrees C provides a better protection than storage at 4 degrees C. (C) 1998 Academic Press.