Guidelines for the use of band-selective radiofrequency pulses in hetero-nuclear NMR: Example of longitudinal-relaxation-enhanced BEST-type 1H-15N correlation experiments
Guidelines for the use of band-selective radiofrequency pulses in hetero-nuclear NMR: Example of longitudinal-relaxation-enhanced BEST-type 1H-15N correlation experiments
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DOI：
10.1016/j.jmr.2009.12.001
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发表时间：
2010-03-01
影响因子：
2.2
通讯作者：
Brutscher, Bernhard
中科院分区：
文献类型：
--
作者：
Lescop, Ewen;Kern, Thomas;Brutscher, Bernhard
频带选择性射频（rf）脉冲为许多生物分子NMR应用提供了强大的光谱工具频带选择性通常通过脉冲成形来实现，其中rf幅度和相位根据数值优化函数进行调制这导致在脉冲持续时间期间复杂的自旋演化轨迹这里我们介绍一些成形脉冲的简化表示，通常用于生物分子NMR光谱学这些简单的方案，包括一套自由演化延迟和理想的RF脉冲，非常好地再现了对标量耦合异核双自旋系统的影响。因此，在复杂的多脉冲序列中最佳使用这种脉冲形状变得简单，如在此对于血管松弛增强的BEST-HSQC和BEST-TROSY实验的实例所证明的，将这些优化的脉冲序列应用于大小范围为8- 10 μ m的几种蛋白质。21 kDa显示（C）2009 Elsevier Inc保留所有权利
Band-selective radiofrequency (rf) Pulses provide powerful spectroscopic tools for many biomolecular NMR applications Band-selectivity is commonly achieved by Pulse shaping where the rf amplitude and phase are modulated according to a numerically optimized function This results in complex spin evolution trajectories during the Pulse duration Here we introduce simplified representations of a number of shaped pulses, commonly used in biomolecular NMR spectroscopy These simple schemes, consisting in a suite of free evolution delays and ideal rf pulses, reproduce astonishingly well the effect on a scalar-coupled hetero-nuclear two-spin system As a consequence, optimal use of such pulse shapes in complex multi-pulse sequences becomes straightforward, as demonstrated here for the example of longitudinal-relaxation-enhanced BEST-HSQC and BEST-TROSY experiments Applications of these optimized pulse sequences to several proteins in the size range of 8-21 kDa are shown (C) 2009 Elsevier Inc All rights reserved