Functional difference of ATP-generating pathways in rooster sperm (Gallus gallus domesticus)
Functional difference of ATP-generating pathways in rooster sperm (Gallus gallus domesticus)
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DOI：
10.1016/j.anireprosci.2021.106843
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发表时间：
2021-09-11
影响因子：
2.2
通讯作者：
Asano, Atsushi
中科院分区：
文献类型：
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作者：
Setiawan, Rangga;Priyadarshana, Chathura;Asano, Atsushi
通过糖酵解和氧化磷酸化产生的三磷酸腺苷（ATP）对于维持精子中鞭毛的运动性是必不可少的;然而，支持受精的主要能量产生途径因物种而异。关于哪些途径维持家禽中ATP的产生和精子活力，存在着思想上的不一致。糖酵解和线粒体氧化有助于鸡精子中的鞭毛运动，但相对依赖于这些途径的运动性和渗透性的内部卵周层仍不清楚。在本研究中，使用各种抑制剂和能量底物来评估厌氧糖酵解和线粒体氧化对鸡精子鞭毛运动、ATP产生和穿透卵周层能力的相对贡献。虽然这两种途径都在不同程度上促进了这些过程，但葡萄糖是鸡精子穿透卵周内层的主要底物。此外，从代谢应激分析的结果表明，有较少的卵周渗透性响应丙酮酸，这不是由于活性氧或细胞内pH值的变化。总体而言，本研究的结果表明，糖酵解和线粒体氧化途径有不同的功能，在鞭毛的运动性和卵周膜的渗透性由公鸡精子。因此，有新的见解，作为本研究的结果到精子的能量生产系统，通过它有利用细胞外代谢底物维持精子受精能力。
Adenosine triphosphate (ATP) production via glycolysis and oxidative phosphorylation is essential for the maintenance of flagellar motility in sperm; however, the primary energy production pathways supporting fertilization vary among species. Inconsistency in thought exists regarding which pathways maintain ATP production and sperm motility in poultry. Glycolysis and mitochondrial oxidation contribute to flagellar motion in chicken sperm, but the relative dependence on these pathways for motility and penetrability into the inner perivitelline layer remains unclear. In the present study, there was use of various inhibitors and energy substrates to evaluate the relative contribution of anaerobic glycolysis and mitochondrial oxidation to chicken sperm flagellar motility, ATP production, and penetrating capacity through the perivitelline layer. Although both pathways contributed to these processes to varying extent, glucose was the primary substrate for sperm penetration into the inner perivitelline layer in chickens. Furthermore, results from metabolic stress analyses indicated that there was less perivitelline penetrability in response to pyruvate that was not due to changes in reactive oxygen species or intracellular pH. Overall, results from the present study indicate glycolysis and mitochondrial oxidation pathways have distinct functions in the flagellar motility and penetrability of the perivitelline membrane by rooster sperm. There, therefore, are new insights as a result of findings in the present study into the energy production system of sperm through which there is utilization of extracellular metabolic substrates for maintaining sperm fertilization capacity.