Transgenic techniques for cell ablation or molecular deletion to investigate functions of astrocytes and other GFAP-expressing cell types.
Transgenic techniques for cell ablation or molecular deletion to investigate functions of astrocytes and other GFAP-expressing cell types.
复制标题
用于细胞消融或分子缺失的转基因技术，以研究星形胶质细胞和其他表达 GFAP 的细胞类型的功能。
DOI：
10.1007/978-1-61779-452-0_35
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Sofroniew,MichaelV
中科院分区：
文献类型：
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作者：
Sofroniew,MichaelV
Genetic tools are enabling the molecular dissection of the functions and mechanisms of many biological processes. Transgenic manipulations provide powerful tools with which to test hypotheses regarding functions of specific cell types and molecules in vivo in combination with different types of experimental models. Various techniques are available that can target genetic manipulations specifically to astrocytes and that are enabling the molecular dissection of astrocyte biology in vivo. This article summarizes procedures and experience from our laboratory using transgenic strategies that enable either the ablation of proliferating astrocytes and related cells, or the deletion of specific molecules selectively from astrocytes, to study the functions of astrocytes and related cell types in health and disease in vivo using different experimental mouse models.
