Gene expression of glucose transporter (GLUT) 1, 3 and 4 in bovine follicle and corpus luteum
Gene expression of glucose transporter (GLUT) 1, 3 and 4 in bovine follicle and corpus luteum
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DOI：
10.1677/joe.1.06210
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发表时间：
2006-01-01
影响因子：
4
通讯作者：
Tetsuka, M
中科院分区：
文献类型：
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作者：
Nishimoto, H;Matsutani, R;Tetsuka, M
葡萄糖是牛卵巢的主要能量底物，充足的葡萄糖供应是维持卵巢活动的必要条件。葡萄糖不能透过质膜，其摄取由许多葡萄糖转运蛋白（GLUT）介导。本研究对牛卵泡和黄体中GLUT 1、3和4基因的表达进行了研究。卵巢获自Holstein × Japanese Black F1小母牛。从卵泡收获颗粒细胞和内膜层，根据其生理状态分为五类：卵泡大小（>= 8-5 mm：优势; < 8-5 mm：从属），卵泡液中雌二醇（E-2）与孕酮的比例（>= 1：E-2活跃;< 1：E-2非活跃）和发情周期阶段（黄体期，卵泡期）。CL也按发情周期的阶段进行了分类。通过实时PCR定量GLUT 1、3和4 mRNA的表达水平。在牛卵泡和CL中检测到GLUT 1和3的mRNA，其水平与经典的GLUT表达器官（如大脑和心脏）中的水平相当。在这些组织中也检测到低得多但可感知的GLUT 4水平。这些GLUT的基因表达表现出组织和阶段特异性模式。尽管在生理条件下有相当大的差异，GLUT 1，3和4的mRNA表达水平相似的下属卵泡，以及占主导地位的E-2-活跃的卵泡在黄体期和卵泡期，而这些GLUT的基因表达显着增加，观察到占主导地位的E-2-非活性卵泡进行闭锁过程。在这些卵泡中，观察到卵泡液中葡萄糖浓度与颗粒细胞中GLUT 1和3 mRNA水平之间存在高度显著的负相关性，这意味着局部葡萄糖环境影响卵泡的葡萄糖摄取。这些结果表明，葡萄糖转运蛋白1和3作为主要的葡萄糖转运蛋白，而葡萄糖转运蛋白4可能发挥支持作用，在牛卵泡和CL。
Glucose is the main energy substrate in the bovine ovary, and a sufficient supply of it is necessary to sustain the ovarian activity. Glucose cannot permeate the plasma membrane, and its uptake is mediated by a number of glucose transporters (GLUT). In the present study, we investigated the gene expression of GLUT1, 3 and 4 in the bovine follicle and corpus luteum (CL). Ovaries were obtained from Holstein x Japanese Black F1 heifers. Granulosa cells and theca interna layers were harvested from follicles classified into five categories by their physiologic status: follicular size ( >= 8-5 mm: dominant; < 8-5 mm: subordinate), ratio of estradiol (E-2) to progesterone in follicular fluid (>= 1: E-2 active;< 1: E-2 inactive), and stage of estrous cycle (luteal phase, follicular phase). CL were also classified by the stage of estrous cycle. Expression levels of GLUT1, 3 and 4 mRNA were quantified by a real-time PCR. The mRNA for GLUT1 and 3 were detected in the bovine follicle and CL at comparable levels to those in classic GLUT-expressing organs such as brain and heart. Much lower but appreciable levels of GLUT4 were also detected in these tissues. The gene expression of these GLUT showed tissue- and stage-specific patterns. Despite considerable differences in physiologic conditions, similar levels of GLUT1, 3 and 4 mRNA were expressed in subordinate follicles as well as dominant E-2-active follicles in both luteal and follicular phases, whereas a notable increase in the gene expression of these GLUT was observed in dominant E-2-inactive follicles undergoing the atretic process. In these follicles, highly significant negative correlations were observed between the concentrations of glucose in follicular fluid and the levels of GLUT1 and 3 mRNA in granulosa cells, implying that the local glucose environment affects glucose uptake of follicles. These results indicate that GLUT1 and 3 act as major transporters of glucose while GLUT4 may play a supporting role in the bovine follicle and CL.