MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND-BINDING PROPERTIES OF TWO ODORANT-BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)
MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS, AND LIGAND-BINDING PROPERTIES OF TWO ODORANT-BINDING PROTEIN GENES FROM Orthaga achatina (BUTLER) (LEPIDOPTERA: PYRALIDAE)
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Orthaga achatina (Butler)（鳞翅目：Pyralidae）两个气味结合蛋白基因的分子特征、表达模式和配体结合特性
DOI：
10.1002/arch.21036
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发表时间：
2012-08-01
影响因子：
2.2
通讯作者：
Mu, Lan-Fang
中科院分区：
文献类型：
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作者：
Liu, Shi-Jing;Liu, Nai-Yong;Mu, Lan-Fang
据推测，昆虫信息素结合蛋白(PBPs)参与性信息素的接受，而一般气味结合蛋白(GOBP)参与包括植物挥发物在内的一般气味的接受。然而，这种功能特异性并不是完全确凿的。在本研究中，两个新的OBP基因的全长序列被分子鉴定为OachPBP1和OachGOBP2，它们来自樟树的重要害虫Othaga Achat ina。QRT-PCR定量分析表明，这两个基因在触角中高度表达，OachPBP1和OachGOBP2在雌雄之间表达相似。这些表达模式与通常提出的PBPs和GOBP的功能是一致的。利用细菌表达系统获得的重组蛋白，通过竞争结合实验进一步研究和比较了这些蛋白的结合特异性。OachPBP1与所有3个推定的性信息素和10个信息素类似物都显示出高度的结合亲和力，支持它在信息素接收中的作用。另一方面，除了与一些植物挥发物结合外，OachGOBP2与性信息素的结合亲和力与OachPBP1相似，甚至更高。因此，我们推测OachGOBP2可能在性信息素的接受性信息素中起作用。此外，植物挥发物法尼醇和法尼烯与OachGOBP2和OachPBP1都有很高的结合力，这表明这些挥发性化学物质对凤眼莲的行为具有调节功能。
It is postulated that insect pheromone-binding proteins (PBPs) are involved in sex pheromone reception, while the general odorant-binding proteins (GOBPs) are involved in reception of the general odorants including plant volatiles. However, this functional specificity is not completely conclusive. In the present study, full-length sequences of two new OBP genes were molecularly identified as OachPBP1 and OachGOBP2 from Orthaga achatina, an important pest of the camphor tree Cinnamomum camphora. Quantification of transcript levels by qRT-PCR showed that the two genes highly expressed in antennae, with OachPBP1 male-biased and OachGOBP2 similar between sexes. These expression patterns are consistent with the generally proposed functions of PBPs and GOBPs. With the recombinant proteins obtained by a bacterial expression system, the binding specificity of these proteins was further investigated and compared using the competitive binding assay. OachPBP1 exhibited high binding affinities with all three putative sex pheromones and 10 pheromone analogs, supporting its role in pheromone reception. On the other hand, in addition to binding with some plant volatiles, OachGOBP2 surprisingly displayed similar or even higher binding affinities with the sex pheromones than OachPBP1. Therefore, we propose that OachGOBP2 might play roles in reception of sex pheromone. Additionally, plant volatiles farnesol and farnesene showed high binding with both OachGOBP2 and OachPBP1, suggesting that these volatile chemicals have regulatory functions in the behavior of O. achatina.