Mast Cells Are Key Promoters of Contact Allergy that Mediate the Adjuvant Effects of Haptens
Mast Cells Are Key Promoters of Contact Allergy that Mediate the Adjuvant Effects of Haptens
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DOI：
10.1016/j.immuni.2011.03.028
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发表时间：
2011-06-24
期刊：
影响因子：
32.4
通讯作者：
Roers, Axel
中科院分区：
文献类型：
--
作者：
Dudeck, Anne;Dudeck, Jan;Roers, Axel
引起过敏性接触性皮炎的致敏环境化合物的一个显著特征是快速诱导固有的炎症反应，这似乎为有效的T细胞启动提供了危险信号。我们建立了肥大细胞缺陷、肥大细胞特异性基因失活的小鼠模型和肥大细胞报告小鼠的活体成像模型，并表明接触性变应原的这些佐剂作用是由肥大细胞和组胺介导的。肥大细胞缺乏导致皮肤树突状细胞向淋巴结的迁移受损，在没有肥大细胞的情况下，接触性超敏反应显著减少。此外，肥大细胞特异性的II10基因失活没有揭示肥大细胞来源的IL-10在调节接触性变态反应中的任何作用。总而言之，我们证明肥大细胞是接触性超敏反应的重要促进剂，从而突出了它们促进对通过皮肤进入的抗原的免疫反应的潜力。
A prominent feature of sensitizing environmental compounds that cause allergic contact dermatitis is the rapid induction of an innate inflammatory response that seems to provide danger signals for efficient T cell priming. We generated mouse models of mast cell deficiency, mast cell-specific gene inactivation, and mast cell reporter mice for intravital imaging and showed that these adjuvant effects of contact allergens are mediated by mast cells and histamine. Mast cell deficiency resulted in impaired emigration of skin DCs to the lymph node and contact hypersensitivity was dramatically reduced in the absence of mast cells. In addition, mast cell-specific inactivation of the II10 gene did not reveal any role for mast cell-derived IL-10 in the regulation of contact allergy. Collectively, we demonstrate that mast cells are essential promoters of contact hypersensitivity, thereby highlighting their potential to promote immune responses to antigens entering via the skin.