Similarity of responses of cultured sertoli cells to cholera toxin and FSH
Similarity of responses of cultured sertoli cells to cholera toxin and FSH
复制标题
培养的支持细胞对霍乱毒素和 FSH 反应的相似性
DOI：
10.1016/0303-7207(76)90090-3
复制
发表时间：
1976
影响因子：
4.1
通讯作者：
J. Dorrington
中科院分区：
文献类型：
--
作者：
I. Fritz;M. Griswold;B. Louis;J. Dorrington
The effects of cholera toxin on the responses of cultured Sertoli cells were compared with those elicited by follicle-stimulating hormone (FSH), andN6O2'-dibutyryl-3',5'-cyclic AMP (bu2cAMP). Addition of FSH or cholera toxin increased cAMP levels. Subsequently there were greater rates of conversion of testosterone to 17β-estradiol, formation of androgen-binding protein (ABP), and incorporation of [3H] thymidine into DNA by Sertoli cells prepared from testes of immature rats and cultured in the presence of either FSH or cholera toxin. Addition of bu2-cAMP also resulted in enhanced rates of formation of ABP, synthesis of 17β-estradiol and synthesis of DNA. Cholera toxin and bu2cAMP elicited changes in morphology of cultured Sertoli cells indistinguishable from those following FSH addition. It is concluded that elevated intracellular cAMP levels can duplicate known actions of FSH on cultured Sertoli cells, but the possible obligatory role of cAMP in mediating FSH actions remains to be evaluated.
