Characterization of the bacterial community in the sediment of a brackish lake with oyster aquaculture.
Characterization of the bacterial community in the sediment of a brackish lake with oyster aquaculture.
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DOI：
10.4265/bio.18.29
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发表时间：
2013
影响因子：
1.2
通讯作者：
S. M. S. Santander‐de Leon-S.-M.-S.-Santander‐de-Leon-1414484914;Suguru Okunishi;Masaki Kihira;M. Nakano;S. Nuñal;M. Hidaka;T. Yoshikawa;H. Maeda
中科院分区：
文献类型：
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作者：
S. M. S. Santander‐de Leon-S.-M.-S.-Santander‐de-Leon-1414484914;Suguru Okunishi;Masaki Kihira;M. Nakano;S. Nuñal;M. Hidaka;T. Yoshikawa;H. Maeda
以白石湖为研究对象，分析了牡蛎养殖、海水和淡水入湖对白石湖沉积物理化性质和细菌群落的影响。物理化学分析表明，沉积物在河口的海洋起源，而较高的有机质负荷和由此产生的厌氧，还原条件的沉积物的内部观察。沉积物中的细菌群落反映了这些沉积物环境：牡蛎养殖场附近的细菌群落包括硫酸盐还原菌（SRB），尽管在所有采样点都发现了硫氧化菌（SRB）。此外，16 S rDNA-变性梯度凝胶电泳（DGGE）得到的带型的相似性下降的比例，从河口的牡蛎养殖场的内部。本研究能够表征微咸水湖泊沉积物中的微生物群落的变化与牡蛎养殖系统通过分子指纹技术，DGGE，与他们的理化特性。
The physicochemical properties and bacterial community in sediments of Lake Shiraishi, a lake with brackish water, were characterized to elucidate the influence of oyster farming and seawater and freshwater inflow. Physicochemical analyses suggested the marine origin of the sediment at the mouth of the lake, while higher organic matter load and the resultant anaerobic, reductive condition of the sediments of the inner part were observed. The bacterial community in the sediments reflects these sediment environments: the bacterial community in the vicinities of oyster farms included sulfate-reducing bacteria (SRB) , although sulfur-oxidizing bacteria (SOB) were found at all the sampling sites. In addition, similarity of the band profiles obtained with 16S ribosomal RNA gene (16S rDNA) -denaturing gradient gel electrophoresis (DGGE) decreased in proportion to the distance from the mouth of the lake to the oyster farms in the inner part. This study was able to characterize the microbial community shift in brackish lake sediments with an oyster aquaculture system through the molecular fingerprinting technique, DGGE, in relation to their physicochemical characteristics.