Zinc oxide nanoparticle inhibits the biofilm formation of Streptococcus pneumoniae
Zinc oxide nanoparticle inhibits the biofilm formation of Streptococcus pneumoniae
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DOI：
10.1007/s10482-017-0930-7
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发表时间：
2018-01-01
影响因子：
2.6
通讯作者：
Tribedi, Prosun
中科院分区：
文献类型：
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作者：
Bhattacharyya, Purnita;Agarwal, Bikash;Tribedi, Prosun
生物膜是微生物细胞的结构联合体，它们生长在活的和非活的表面上，并被分泌的聚合物包围。细菌生物膜感染已成为最重要的公共卫生问题，因为生物膜生长细胞对抗生素和宿主免疫防御都具有高度抗药性。氧化锌纳米颗粒已被报道为一种潜在的抗菌剂，因此，在本研究中，我们评估了氧化锌纳米颗粒对肺炎链球菌的抗菌和抗生物被膜活性，肺炎链球菌是一种重要的致病原因。纳米氧化锌对肺炎链球菌具有较强的抗菌活性，其MIC值为40微克/毫升，通过结晶紫法、显微镜观察、蛋白质计数、EPS分泌等一系列实验，评价了纳米氧化锌对肺炎链球菌的抑制作用。结果表明，亚MIC剂量的氧化锌纳米颗粒对肺炎链球菌具有显著的抗生物被膜作用，最大生物被膜衰减量为12微克/毫升，说明纳米氧化锌颗粒可以作为一种潜在的微生物生物被膜抑制剂。
Biofilms are structured consortia of microbial cells that grow on living and non living surfaces and surround themselves with secreted polymers. Infections with bacterial biofilms have emerged as a foremost public health concern because biofilm growing cells can be highly resistant to both antibiotics and host immune defenses. Zinc oxide nanoparticles have been reported as a potential antimicrobial agent, thus, in the current study, we have evaluated the antimicrobial as well as antibiofilm activity of zinc oxide nanoparticles against the bacterium Streptococcus pneumoniae which is a significant cause of disease. Zinc oxide nanoparticles showed strong antimicrobial activity against S. pneumoniae, with an MIC value of 40 mu g/ml. Biofilm inhibition of S. pneumoniae was also evaluated by performing a series of experiments such as crystal violet assay, microscopic observation, protein count, EPS secretion etc. using sub-MIC concentrations (3, 6 and 12 mu g/ml) of zinc oxide nanoparticles. The results showed that the sub-MIC doses of zinc oxide nanoparticles exhibited significant anti-biofilm activity against S. pneumoniae, with maximum biofilm attenuation found at 12 mu g/ml. Taken together, the results indicate that zinc oxide nanoparticles can be considered as a potential agent for the inhibition of microbial biofilms.