The effect of Vaccinium uliginosum on rabbit retinal structure and light-induced function damage
The effect of Vaccinium uliginosum on rabbit retinal structure and light-induced function damage
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DOI：
10.1007/s11655-011-0901-1
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发表时间：
2012-03
影响因子：
2.9
通讯作者：
Lan Yin;Y. Pi;Mao‐nian Zhang
中科院分区：
文献类型：
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作者：
Lan Yin;Y. Pi;Mao‐nian Zhang
目的研究越桔(Vaccdium uliginosumL.，VU)对兔视网膜光损伤后视网膜电图(ERG)和视网膜病理改变的影响。方法将28只青紫蓝兔随机分为4组：预先给药组(A组)、伤后给药组(B组)、光损伤不给药组(C组)和空白对照组(D组)。A组静脉注射VU匀浆4.8g/(kg.d)，4周后，A、B、C组按国际临床视觉电生理学会(ISCEV)的标准记录ERG。除D组外，其余各组均接受强光照射。此后，A组停止接受VU治疗，B组开始接受VU治疗。分别于术后1d、1周、2周记录各组大鼠的ERG。在整个过程中，没有饲喂VU的组喂以生理盐水。结果(1)VU喂养4周后，A组大鼠ERG潜伏期缩短，波幅增大。强光照射后，各损伤组潜伏期延长，波幅降低，但各时间点比较，A组优于C组，随着VU的积聚，B组ERG有所改善，最终各项测定值均优于C组。(2)D组视网膜组织学正常，各层排列整齐，C组视网膜排列紊乱。与C组相比，A、B组损伤较轻。C组ONL厚度较其他组明显变薄(P=0.000)，A、B组虽较D组薄，但较C组厚，且A组较B组厚。结论VU可减轻正常昼夜节律暴露下兔视网膜的损伤，减轻预先用光对视网膜的损伤，修复视网膜的光损伤。
ObjectiveTo study the effect ofVaccinium uliginosumL., (VU) on the electroretinogram (ERG) and retinal pathological changes in rabbits after light-induced damage.MethodsTwenty-eight Chinchilla rabbits were randomly divided into four groups: administration beforehand (A), administration after injury (B), light injury without administration (C), and blank (D) groups. After a 4-week administration of VU homogenate at 4.8 g/(kg·d) once a day in group A, ERG in groups A, B and C were recorded according to the standards set by the International Society for Clinical Electrophysiology of Vision (ISCEV). Except for group D, the groups were then exposed to strong light. Just after that, group A stopped receiving VU treatment and group B started to receive it. Then ERGs in all groups were recorded after 1 day, 1 week, and 2 weeks. Throughout the whole process groups which were not fed with VU were fed with normal saline. Finally, the tissues and structures of all the groups were observed and the thickness of the outer nuclear layers (ONL) was measured.Results(1) After 4-week feeding with VU, the latency time of ERG in group A became shorter than those in the other groups and the amplitude increased. After being exposed to strong light, the latency time lengthened and amplitude decreased in all the injury groups, but comparing at each time point, the measured values in group A were better than those in group C. With the accumulation of VU, the ERG in group B improved, and finally, all of the detected values became better than those in group C. (2) Retinae in group D were normal in histology and the layers were in order but those in group C became disarranged. The injuries in groups A and B were minor compared with those in group C. The thickness of the ONL in group C was significantly thinner than in the other groups (P=0.000), and that in groups A and B was thicker than that in group C, although thinner than in group D. That in group A was thicker than in group B.ConclusionsVU can relieve the injury to rabbit retinae exposed to normal day and night rhythm, alleviate the harm caused by light when used beforehand, and repair the light damage to the retina.