VarLOCK - sequencing independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance
VarLOCK - sequencing independent, rapid detection of SARS-CoV-2 variants of concern for point-of-care testing, qPCR pipelines and national wastewater surveillance
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VarLOCK - 独立于测序、快速检测关注点检测、qPCR 管道和国家废水监测的 SARS-CoV-2 变体
DOI：
10.1101/2022.01.06.21268555
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Nan X
中科院分区：
文献类型：
--
作者：
Nan X
2019冠状病毒病大流行表明需要快速分子诊断。疫苗接种计划可以提供保护，促进社会开放，但新出现的和现有的病毒变种能够逃避免疫系统危及其效力。因此，对关注变体（VOC）的有效监测非常重要。与单独的基因组测序相比，快速和特异性的分子诊断可以提供速度和覆盖率优势，有利于公共卫生反应并促进VOC遏制。在这里，我们扩展了最近开发的SARS-CoV-2 CRISPR-Cas检测技术（SHERMOND），以提供快速和灵敏的SARS-CoV-2 VOC鉴别，可用于护理点，在小型或大型测试设施的管道中实施，甚至确定混合人口水平废水样本中VOC的比例。这项技术补充了测序工作，可以方便快速地识别受VOC感染的个体，以帮助打破感染链。我们显示了我们的变异检测（变异特异性SHERRYST）的多个特定突变的S基因的SARS冠状病毒-2和验证样品从卡迪夫大学测试服务的优化。我们还展示了Varadox对SARS-CoV-2变体的国家废水监测的适用性，以及该技术对新出现的VOCs的快速适应性。
The COVID-19 pandemic demonstrated the need for rapid molecular diagnostics. Vaccination programs can provide protection and facilitate the opening of society, but newly emergent and existing viral variants capable of evading the immune system endanger their efficacy. Effective surveillance for Variants of Concern (VOC) is therefore important. Rapid and specific molecular diagnostics can provide speed and coverage advantages compared to genomic sequencing alone, benefitting the public health response and facilitating VOC containment. Here we expand the recently developed SARS-CoV-2 CRISPR-Cas detection technology (SHERLOCK) to provide rapid and sensitive discrimination of SARS-CoV-2 VOCs that can be used at point of care, implemented in the pipelines of small or large testing facilities, and even determine the proportion of VOCs in pooled population-level wastewater samples. This technology complements sequencing efforts to allow facile and rapid identification of individuals infected with VOCs to help break infection chains. We show the optimisation of our VarLOCK assays (Variant-specific SHERLOCK) for multiple specific mutations in the S gene of SARS-CoV-2 and validation with samples from the Cardiff University Testing Service. We also show the applicability of VarLOCK to national wastewater surveillance of SARS-CoV-2 variants and the rapid adaptability of the technique for new and emerging VOCs.
影响因子：
3.7
作者：
Schermer B;Fabretti F;Damagnez M;Di Cristanziano V;Heger E;Arjune S;Tanner NA;Imhof T;Koch M;Ladha A;Joung J;Gootenberg JS;Abudayyeh OO;Burst V;Zhang F;Klein F;Benzing T;Müller RU
通讯作者：
Müller RU