Relationship of lipoprotein(a) molar concentrations and mass according to lipoprotein(a) thresholds and apolipoprotein(a) isoform size
Relationship of lipoprotein(a) molar concentrations and mass according to lipoprotein(a) thresholds and apolipoprotein(a) isoform size
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DOI：
10.1016/j.jacl.2018.07.003
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发表时间：
2018-10-01
影响因子：
4.4
通讯作者：
Marcovina, Santica M.
中科院分区：
文献类型：
--
作者：
Tsimikas, Sotirios;Fazio, Sergio;Marcovina, Santica M.
背景：脂蛋白(A)[Lp(A)]被报道为Lp(A)颗粒质量(mg/dL)或载脂蛋白(A)[apo(A)]的摩尔浓度(nmoL/L)，被认为是金标准。目的：在不同的Lp(A)水平阈值和载脂蛋白(A)亚型大小的背景下，量化Lp(A)摩尔浓度和Lp(A)质量之间的关系。方法：在所有样本中，在西北脂肪代谢和糖尿病研究实验室(NLMDRL)测定Lp(A)摩尔浓度和载脂蛋白(A)亚型大小。Lp(A)质量水平是在加州大学圣地亚哥分校(UCSD)(1635个样本)通过5种市面上可用的分析方法测定的：Denka 1和Denka 2(各80个样本)，2个比浊法(分别为2545个和2673个样本)，以及酶联免疫吸附分析(2605个样本)。结果：NLMDRL/UCSD、NLMDRL/Denka1和NLMDRL/Denka2的平均比值(SD)分别为2.42(1.25)、1.64(0.18)和2.02(0.22)。Lp(A)和Lp(A)/L的NLMDRL/UCSD、NLMDRL/Denka1和NLMDRL/Denka2的比值分别为1.82、1.52和1.87，而Lp(A)和Lt；125nmol/L的比值分别为2.80、1.89和2.24。不同化验方法之间的单一换算系数是不合适的。这些数据支持将Lp(A)质量检测转变为摩尔浓度，以改进Lp(A)介导的风险的诊断和临床解释。(三)2018年全国油脂协会。版权所有。
BACKGROUND: Lipoprotein(a) [Lp(a)] is reported as Lp(a) particle mass (mg/dL) or molar concentration of apolipoprotein(a) [apo(a)] (nmol/L), which is considered the gold standard. Values are often converted from one measurement to the other but the validity of this is unknown.OBJECTIVES: To quantify the relationship between Lp(a) molar concentration and Lp(a) mass in the context of various Lp(a) level thresholds and apo(a) isoform size.METHODS: In all samples, Lp(a) levels in molar concentration and apo(a) isoform size were determined at the Northwest Lipid Metabolism and Diabetes Research Laboratories (NLMDRL). Lp(a) mass levels were determined at the University of California, San Diego (UCSD) (1635 samples), by 5 commercially available assays: Denka 1 and Denka 2 (each 80 samples), 2 turbidimetric assays (2545 and 2673 samples, respectively), and an enzyme-linked immunosorbent assay (2605 samples). The ratios between Lp(a) molar concentration and mass (eg, nmol/L/mg/dL) were calculated and related to apo(a) isoform size.RESULTS: The mean (SD) ratios for NLMDRL/UCSD, NLMDRL/Denka1, and NLMDRL/Denka2 were 2.42 (1.25), 1.64 (0.18), and 2.02 (0.22), respectively. The ratios for NLMDRL/UCSD, NLMDRL/Denka1, and NLMDRL/Denka2 increased by Lp(a) cutoffs, with ratios of 1.82, 1.52, and 1.87, respectively, for Lp(a) < 75 nmol/L and 2.80, 1.89, and 2.24, respectively, for Lp(a) > 125 nmol/L. For the commercial turbidimetric assays and enzyme-linked immunosorbent assay, the ratios ranged from 5.CONCLUSIONS: Lp(a) molar/mass ratios are threshold, method, and isoform dependent. A single conversion factor between assays is not appropriate. These data support the transition of Lp(a) mass assays to molar concentration to improve diagnostic and clinical interpretation of Lp(a)-mediated risk. (C) 2018 National Lipid Association. All rights reserved.