Analysis of Gene Expression in a Human-derived Glial Cell Line Exposed to 2.45 GHz Continuous Radiofrequency Electromagnetic Fields
Analysis of Gene Expression in a Human-derived Glial Cell Line Exposed to 2.45 GHz Continuous Radiofrequency Electromagnetic Fields
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DOI：
10.1269/jrr.10116
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Miyakoshi, Junji
中科院分区：
文献类型：
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作者：
Sakurai, Tomonori;Kiyokawa, Tomoko;Miyakoshi, Junji
射频电磁场/人源性细胞/DNA微阵列/聚合酶链反应。移动电话使用的增加引起了公众对射频场潜在健康风险的关注。我们利用DNA微阵列技术研究了在特定吸收率（SAR）为1、5和10 W/kg的射频场（2.45 GHz，连续波）下暴露1、4和24小时对正常人神经胶质细胞系SVGp12基因表达的影响。微阵列分析显示23个指定基因点和5个非指定基因点是潜在的改变基因点。通过逆转录-聚合酶链反应对23个指定基因点中的22个基因进行进一步分析以验证芯片结果，未观察到基因表达的显著变化。在本研究的实验条件下，我们没有发现暴露于射频场影响SVGp12细胞基因表达的证据。
Radiofrequency electromagnetic fields/Human-derived cells/DNA microarray/Polymerase chain reaction.The increasing use of mobile phones has aroused public concern regarding the potential health risks of radiofrequency (RF) fields. We investigated the effects of exposure to RF fields (2.45 GHz, continuous wave) at specific absorption rate (SAR) of 1, 5, and 10 W/kg for 1, 4, and 24 h on gene expression in a normal human glial cell line, SVGp12, using DNA microarray. Microarray analysis revealed 23 assigned gene spots and 5 non-assigned gene spots as prospective altered gene spots. Twenty-two genes out of the 23 assigned gene spots were further analyzed by reverse transcription-polymerase chain reaction to validate the results of microarray, and no significant alterations in gene expression were observed. Under the experimental conditions used in this study, we found no evidence that exposure to RF fields affected gene expression in SVGp12 cells.